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Literature summary extracted from

  • Eprintsev, A.T.; Semenova, E.V.; Popov, V.N.
    Induction of aconitate hydratase in hepatocytes of starving rats (2002), Biochemistry (Moscow), 67, 795-801.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.3 cysteine enzyme activity increases 2-4fold in presence of both Fe2+ and cysteine. 0.1-1 mM Fe2+ and 0.05-0.5 mM cysteine Rattus rattus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.3 D-glucose 1-phosphate
-
 Rattus rattus
4.2.1.3 D-glucose 6-phosphate
-
 Rattus rattus
4.2.1.3 fumarate
-
 Rattus rattus
4.2.1.3 glyoxylate
-
 Rattus rattus
4.2.1.3 H2O2 2.3 mM, 90-95% inhibition Rattus rattus
4.2.1.3 malate
-
 Rattus rattus
4.2.1.3 oxaloacetate
-
 Rattus rattus
4.2.1.3 succinate
-
 Rattus rattus
4.2.1.3 trans-aconitate
-
 Rattus rattus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.1.3 cytosol 79% cytosolic enzyme and 21% mitochondrial enzyme for control animals, 83% cytosolic enzyme and 17% mitochondrial enzyme for starving animals Rattus rattus 5829
-
4.2.1.3 mitochondrion 79% cytosolic enzyme and 21% mitochondrial enzyme for control animals, 83% cytosolic enzyme and 17% mitochondrial enzyme for starving animals Rattus rattus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.3 Fe2+ enzyme activity increases 2-4fold in presence of both Fe2+ and cysteine. 0.1-1 mM Fe2+ and 0.05-0.5 mM cysteine Rattus rattus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.3 41000
-
 2 * 41000, mitochondrial enzyme, SDS-PAGE Rattus rattus
4.2.1.3 45000
-
 2 * 45000, cytosolic enzyme, SDS-PAGE Rattus rattus
4.2.1.3 84000
-
 mitochondrial enzyme, gel filtration Rattus rattus
4.2.1.3 96000
-
 cytosolic enzyme, gel filtration Rattus rattus

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.3 Rattus rattus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.3 liver
-
 Rattus rattus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.3 6.13
-
 mitochondrial enzyme Rattus rattus
4.2.1.3 11.1
-
 cytosolic enzyme Rattus rattus

Storage Stability

EC Number Storage Stability Organism
4.2.1.3 4°C, activity of the highly purified cytosolic enzyme decreases by 15-20% during the first 24 h of storage and then by 5% every 24 h, the mitochondrial enzyme decreases 35-40% during the first 24 h and then by 15% every 24 h Rattus rattus

Subunits

EC Number Subunits Comment Organism
4.2.1.3 dimer 2 * 41000, mitochondrial enzyme, SDS-PAGE Rattus rattus
4.2.1.3 dimer 2 * 45000, cytosolic enzyme, SDS-PAGE Rattus rattus

Synonyms

EC Number Synonyms Comment Organism
4.2.1.3 AH
-
 Rattus rattus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.3 15
-
-
 Rattus rattus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.3 7.8 8
-
 Rattus rattus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.3 0.14
-
 glyoxylate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 0.33
-
 succinate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 0.4
-
 fumarate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 0.42
-
 glyoxylate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 0.51
-
 fumarate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 0.61
-
 succinate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 0.81
-
 D-glucose 1-phosphate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 1.03
-
 oxaloacetate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 1.14
-
 D-glucose 6-phosphate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 1.37
-
 trans-aconitate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 1.81
-
 malate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 1.9
-
 D-glucose 1-phosphate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 2.35
-
 D-glucose 6-phosphate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 2.8
-
 oxaloacetate cytosolic enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 2.94
-
 malate mitochondrial enzyme, with isocitrate as substrate Rattus rattus
4.2.1.3 3.41
-
 trans-aconitate cytosolic enzyme, with isocitrate as substrate Rattus rattus