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Literature summary extracted from

  • Moiseeva, O.V.; Belova, O.V.; Solyanikova, I.P.; Schlomann, M.; Golovleva, L.A.
    Enzymes of a new modified ortho-pathway utilizing 2-chlorophenol in Rhodococcus opacus 1CP (2001), Biochemistry (Moscow), 66, 548-555.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.5.1.7 0.1429
-
 2-chloro-cis,cis-muconate
-
 Rhodococcus opacus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.7 additional information Rhodococcus opacus key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway ?
-
 ?
5.5.1.7 additional information Rhodococcus opacus 1CP key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.7 Rhodococcus opacus
-
 strain1CP
-
5.5.1.7 Rhodococcus opacus 1CP
-
 strain1CP
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.7
-
 Rhodococcus opacus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.7 18
-
-
 Rhodococcus opacus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.7 2-Chloro-cis,cis-muconate
-
 Rhodococcus opacus 5-Chloromuconolactone
-
 ?
5.5.1.7 2-Chloro-cis,cis-muconate
-
 Rhodococcus opacus 1CP 5-Chloromuconolactone
-
 ?
5.5.1.7 additional information no activity with cis,cis-muconate, 3-chloromuconate, 3-methylmuconate and 2,4-dichloromuconate Rhodococcus opacus ?
-
 ?
5.5.1.7 additional information key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway Rhodococcus opacus ?
-
 ?
5.5.1.7 additional information no activity with cis,cis-muconate, 3-chloromuconate, 3-methylmuconate and 2,4-dichloromuconate Rhodococcus opacus 1CP ?
-
 ?
5.5.1.7 additional information key enzyme in a modified ortho-pathway in cells utilizing 2-chlorophenol via a 3-chlorocatechol branch of a modified ortho-pathway Rhodococcus opacus 1CP ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.7 CMCI
-
 Rhodococcus opacus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.5.1.7 65
-
-
 Rhodococcus opacus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.5.1.7 171000
-
 2-chloro-cis,cis-muconate
-
 Rhodococcus opacus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5.1.7 5.7
-
 in borate-phosphate-acetate buffer Rhodococcus opacus
5.5.1.7 6.2
-
 in bis-Tris-HCl and Tris-HCl buffer Rhodococcus opacus