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Literature summary extracted from

  • Sheng, Y.; Ip, H.; Liu, J.; Davidson, A.; Bognar, A.L.
    Binding of ATP as well as tetrahydrofolate induces conformational changes in Lactobacillus casei folylpolyglutamate synthetase in solution (2003), Biochemistry, 42, 1537-1543.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.2.17 D345C more than 85% of the wild type activity, with 5,10-methylene-5,6,7,8-tetrahydrofolic acid as substrate Lacticaseibacillus casei
6.3.2.17 K172C more than 85% of the wild type activity, with 5,10-methylene-5,6,7,8-tetrahydrofolic acid as substrate Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.2.17 0.03
-
 5,10-methylene-5,6,7,8-tetrahydrofolic acid pH 9.75 Lacticaseibacillus casei
6.3.2.17 1
-
 (6RS)-5,6,7,8-tetrahydrofolic acid pH 9.75 Lacticaseibacillus casei
6.3.2.17 3
-
 ATP pH 9.75 Lacticaseibacillus casei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.17 ATP + tetrahydrofolate-Glu + L-glutamate Lacticaseibacillus casei
-
 ADP + phosphate + tetrahydrofolyl-Glu2
-
 ?
6.3.2.17 additional information Lacticaseibacillus casei the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Lacticaseibacillus casei
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.2.17 0.16
-
 D345C mutant, pH 9.75 Lacticaseibacillus casei
6.3.2.17 0.176
-
 double mutant enzyme, pH 9.75 Lacticaseibacillus casei
6.3.2.17 0.18
-
 K172C mutant, pH 9.75 Lacticaseibacillus casei
6.3.2.17 0.2
-
 wild type, pH 9.75 Lacticaseibacillus casei

Storage Stability

EC Number Storage Stability Organism
6.3.2.17 -70ºC, 20% DMSO, stable Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + (6RS)-5,6,7,8-tetrahydrofolic acid + L-glutamate not as effective as 5,10-methylene-5,6,7,8-tetrahydrofolic acid Lacticaseibacillus casei ADP + phosphate + (6S)-5,6,7,8-tetrahydrofolyl-Glu
-
 ?
6.3.2.17 ATP + 5,10-methylene-5,6,7,8-tetrahydrofolic acid + L-glutamate
-
 Lacticaseibacillus casei ADP + phosphate + 5,10-methylene-5,6,7,8-tetrahydrofolyl-Glu
-
 ?
6.3.2.17 ATP + tetrahydrofolate-Glu + L-glutamate
-
 Lacticaseibacillus casei ADP + phosphate + tetrahydrofolyl-Glu2
-
 ?
6.3.2.17 additional information the enzyme metabolizes tetrahydrofolates to long chain polyglutamates within the cytosol or mitochondria of the cells as a mechanism to trap them within the cell Lacticaseibacillus casei ?
-
 ?