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Literature summary extracted from

  • Bovee, M.L.; Pierce, M.A.; Francklyn, C.S.
    Induced fit and kinetic mechanism of adenylation catalyzed by Escherichia coli threonyl-tRNA synthetase (2003), Biochemistry, 42, 15102-15113.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.3 overexpression in strain IBPC6881 as His-tagged enzyme Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.3 W434Y reduced activity, Trp434 is involved in conformational changes during substrate binding Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.1.1.3 additional information
-
 additional information kinetics and kinetic mechanism Escherichia coli
6.1.1.3 0.201
-
 L-threonine wild-type enzyme, pH 7.2, 37°C Escherichia coli
6.1.1.3 0.267
-
 ATP wild-type enzyme, pH 7.2, 37°C Escherichia coli
6.1.1.3 0.387
-
 ATP mutant enzyme W434Y, pH 7.2, 37°C Escherichia coli
6.1.1.3 0.897
-
 L-threonine mutant enzyme W434Y, pH 7.2, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.3 Mg2+ required Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr Escherichia coli
-
 AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.3 recombinant His-tagged enzyme from strain IBPC6881, to homogeneity Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.3 ATP + L-threonine + tRNAThr = AMP + diphosphate + L-threonyl-tRNAThr induced fit, Trp434 is involved in conformational changes during substrate binding Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.3 ATP + L-threonine + tRNAThr
-
 Escherichia coli AMP + diphosphate + L-threonyl-tRNAThr
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.3 Synthetase, threonyl-transfer ribonucleate
-
 Escherichia coli
6.1.1.3 Threonine translase
-
 Escherichia coli
6.1.1.3 Threonine--tRNA ligase
-
 Escherichia coli
6.1.1.3 Threonine-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-ribonucleic synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-transfer ribonucleic acid synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.3 Threonyl-tRNA synthetase
-
 Escherichia coli
6.1.1.3 ThrRS
-
 Escherichia coli
6.1.1.3 TRS
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.1.1.3 30
-
 L-threonine mutant enzyme W434Y, pH 7.2, 37°C Escherichia coli
6.1.1.3 42
-
 ATP mutant enzyme W434Y, pH 7.2, 37°C Escherichia coli
6.1.1.3 90
-
 ATP wild-type enzyme, pH 7.2, 37°C Escherichia coli
6.1.1.3 90
-
 L-threonine wild-type enzyme, pH 7.2, 37°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.3 ATP
-
 Escherichia coli