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Literature summary extracted from

  • O'Brien, G.; Quinsey, N.S.; Whisstock, J.C.; Pike, R.N.
    Importance of the prime subsites of the C1s protease of the classical complement pathway for recognition of substrates (2003), Biochemistry, 42, 14939-14945.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.42 additional information
-
 2-[5-Amino-2-(4-fluoro-phenyl)-6-oxo-6H-pyrimidin-1-yl]-N-(1-benzyl-2-oxo-2-thiazol-2-yl-ethyl)-acetamide K0.5-values Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.42 Homo sapiens
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.42 2-aminobenzoyl-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(dinitrophenyl)-NH2 + H2O
-
 Homo sapiens 2-aminobenzoyl-Gly-Leu-Gln-Arg + Ala-Leu-Glu-Ile-Lys(dinitrophenyl)-NH2
-
 ?
3.4.21.42 2-aminobenzoyl-Ser-Leu-Gly-Arg-Lys-Ile-Gln-Ile-Lys(dinitrophenyl)-NH2 + H2O
-
 Homo sapiens 2-aminobenzoyl-Ser-Leu-Gly-Arg + Lys-Ile-Gln-Ile-Lys(dinitrophenyl)-NH2
-
 ?
3.4.21.42 benzyloxycarbonyl-Gly-Leu-Gln-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-Leu-Gln-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.42 benzyloxycarbonyl-Ser-Leu-Gly-Arg-4-methylcoumaryl-7-amide + H2O
-
 Homo sapiens benzyloxycarbonyl-Ser-Leu-Gly-Arg + 7-amino-4-methylcoumarin
-
 ?
3.4.21.42 complement component C2 + H2O the P1'-P4' substrate residues of C2 confer greater affinity of substrate for enzyme and also induce a sigmoidal dependence of enzyme velocity on substrate concentration. The substrate gives rise to homotropic positive cooperative behavior in the enzyme Homo sapiens complement component C2a + complement component C2b
-
 ?
3.4.21.42 complement component C4 + H2O the P1'-P4' substrate residues of C4 confer greater affinity of substrate for enzyme and also induce a sigmoidal dependence of enzyme velocity on substrate concentration. The substrate gives rise to homotropic positive cooperative behavior in the enzyme Homo sapiens complement component C4a + complement component C4b
-
 ?
3.4.21.42 GLQRA + H2O
-
 Homo sapiens GLQR + Ala
-
 ?
3.4.21.42 GLQRAAEI + H2O
-
 Homo sapiens GLQR + AAEI
-
 ?
3.4.21.42 GLQRAEEI + H2O
-
 Homo sapiens GLQR + AEEI
-
 ?
3.4.21.42 GLQRAFEI + H2O
-
 Homo sapiens GLQR + AFEI
-
 ?
3.4.21.42 GLQRAHEI + H2O
-
 Homo sapiens GLQR + AHEI
-
 ?
3.4.21.42 GLQRAL + H2O
-
 Homo sapiens GLQR + Ala-Leu
-
 ?
3.4.21.42 GLQRALAI + H2O
-
 Homo sapiens GLQR + ALAI
-
 ?
3.4.21.42 GLQRALE + H2O
-
 Homo sapiens GLQR + ALE
-
 ?
3.4.21.42 GLQRALEA + H2O
-
 Homo sapiens GLQR + ALEA
-
 ?
3.4.21.42 GLQRALEE + H2O
-
 Homo sapiens GLQR + ALEE
-
 ?
3.4.21.42 GLQRALEF + H2O
-
 Homo sapiens GLQR + ALEF
-
 ?
3.4.21.42 GLQRALEH + H2O
-
 Homo sapiens GLQR + ALEH
-
 ?
3.4.21.42 GLQRALEI + H2O
-
 Homo sapiens GLQR + ALEI
-
 ?
3.4.21.42 GLQRALEK + H2O
-
 Homo sapiens GLQR + ALEK
-
 ?
3.4.21.42 GLQRALEL + H2O
-
 Homo sapiens GLQR + ALEL
-
 ?
3.4.21.42 GLQRALEM + H2O
-
 Homo sapiens GLQR + ALEM
-
 ?
3.4.21.42 GLQRALEP + H2O
-
 Homo sapiens GLQR + ALEP
-
 ?
3.4.21.42 GLQRALEQ + H2O
-
 Homo sapiens GLQR + ALEQ
-
 ?
3.4.21.42 GLQRALET + H2O
-
 Homo sapiens GLQR + ALET
-
 ?
3.4.21.42 GLQRALFI + H2O
-
 Homo sapiens GLQR + ALFI
-
 ?
3.4.21.42 GLQRALHI + H2O
-
 Homo sapiens GLQR + ALHI
-
 ?
3.4.21.42 GLQRALKI + H2O
-
 Homo sapiens GLQR + ALKI
-
 ?
3.4.21.42 GLQRALLI + H2O
-
 Homo sapiens GLQR + ALLI
-
 ?
3.4.21.42 GLQRALMI + H2O
-
 Homo sapiens GLQR + ALMI
-
 ?
3.4.21.42 GLQRALPI + H2O
-
 Homo sapiens GLQR + ALPI
-
 ?
3.4.21.42 GLQRALQI + H2O
-
 Homo sapiens GLQR + ALQI
-
 ?
3.4.21.42 GLQRALTI + H2O
-
 Homo sapiens GLQR + ALTI
-
 ?
3.4.21.42 GLQRAMEI + H2O
-
 Homo sapiens GLQR + AMEI
-
 ?
3.4.21.42 GLQRAPEI + H2O
-
 Homo sapiens GLQR + APEI
-
 ?
3.4.21.42 GLQRAQEI + H2O
-
 Homo sapiens GLQR + AQEI
-
 ?
3.4.21.42 GLQRATEI + H2O
-
 Homo sapiens GLQR + ATEI
-
 ?
3.4.21.42 GLQRELEI + H2O
-
 Homo sapiens GLQR + ELEI
-
 ?
3.4.21.42 GLQRFLEI + H2O
-
 Homo sapiens GLQR + FLEI
-
 ?
3.4.21.42 GLQRHLEI + H2O
-
 Homo sapiens GLQR + HLEI
-
 ?
3.4.21.42 GLQRKLEI + H2O
-
 Homo sapiens GLQR + KLEI
-
 ?
3.4.21.42 GLQRLLEI + H2O
-
 Homo sapiens GLQR + LLEI
-
 ?
3.4.21.42 GLQRMLEI + H2O
-
 Homo sapiens GLQR + MLEI
-
 ?
3.4.21.42 GLQRQLEI + H2O
-
 Homo sapiens GLQR + QLEI
-
 ?
3.4.21.42 GLQRTLEI + H2O
-
 Homo sapiens GLQR + TLEI
-
 ?
3.4.21.42 additional information importance of the prime subsites of the C1s protease for recognition of substrates no cleavage of GLQRPLEI and GLQRAKEI Homo sapiens ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.42 0.0000189
-
 GLQRAPEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000205
-
 GLQRELEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000394
-
 GLQRA pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000421
-
 GLQRAEEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000702
-
 GLQRLLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000702
-
 GLQRQLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000762
-
 GLQRKLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000978
-
 GLQRALEL pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000978
-
 GLQRALEQ pH 7.4, 37°C Homo sapiens
3.4.21.42 0.0000978
-
 GLQRALEE pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000119
-
 GLQRAMEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000136
-
 GLQRALKI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000147
-
 GLQRATEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000155
-
 GLQRFLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000169
-
 GLQRAAEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000171
-
 GLQRALEM pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000183
-
 GLQRAQEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000205
-
 GLQRALEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00021
-
 2-aminobenzoyl-Gly-Leu-Gln-Arg-Ala-Leu-Glu-Ile-Lys(dinitrophenyl)-NH2 pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00022
-
 GLQRALEF pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000239
-
 GLQRMLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000244
-
 GLQRALEH pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000319
-
 GLQRALE pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000323
-
 GLQRAQEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00033
-
 GLQRAFEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000392
-
 GLQRALEA pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000415
-
 GLQRHLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00044
-
 GLQRALET pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000683
-
 GLQRAL pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000807
-
 GLQRALEP pH 7.4, 37°C Homo sapiens
3.4.21.42 0.000867
-
 GLQRALEK pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00139
-
 2-aminobenzoyl -Ser-Leu-Gly-Arg-Lys-Ile-Gln-Ile-Lys(dinitrophenyl)-NH2 pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00155
-
 GLQRTLEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00227
-
 GLQRALMI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00232
-
 GLQRAQEI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00244
-
 GLQRALPI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00274
-
 GLQRALAI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00279
-
 GLQRALTI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00288
-
 GLQRALFI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00296
-
 GLQRALLI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.00821
-
 GLQRALHI pH 7.4, 37°C Homo sapiens
3.4.21.42 0.38
-
 benzyloxycarbonyl-Gly-Leu-Gln-Arg-4-methylcoumaryl-7-amide pH 7.4, 37°C Homo sapiens
3.4.21.42 0.5
-
 benzyloxycarbonyl-Ser-Leu-Gly-Arg-4-methylcoumaryl-7-amide pH 7.4, 37°C Homo sapiens