BRENDA - Enzyme Database

A gene cluster responsible for alkylaldoxime metabolism coexisting with nitrile hydratase and amidase in Rhodococcus globerulus A-4

Xie, S.X.; Kato, Y.; Komeda, H.; Yoshida, S.; Asano, Y.; Biochemistry 42, 12056-12066 (2003)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.99.1.5
2-mercaptoethanol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.13fold
Rhodococcus globerulus
4.99.1.5
cysteamine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 3.93fold
Rhodococcus globerulus
4.99.1.5
duroquinone
activates
Rhodococcus globerulus
4.99.1.5
FAD
1 mM, 1.2fold activation in presence of Na2S, 4.5fold in absence of Na2S
Rhodococcus globerulus
4.99.1.5
FMN
1 mM, 1.7fold activation in presence of Na2S, 2.4fold in absence of Na2S
Rhodococcus globerulus
4.99.1.5
L-cysteine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.39fold
Rhodococcus globerulus
4.99.1.5
additional information
not activated by NaHSO3, Na2SO4, NaHSO4 or Na2S2O7
Rhodococcus globerulus
4.99.1.5
Na2S
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.35fold
Rhodococcus globerulus
4.99.1.5
Na2S2O4
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.13fold
Rhodococcus globerulus
4.99.1.5
Na2S2O5
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 5.84fold
Rhodococcus globerulus
4.99.1.5
Na2SO3
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 7.51fold
Rhodococcus globerulus
4.99.1.5
reducing reagent
requirement, activates
Rhodococcus globerulus
4.99.1.5
riboflavin
1 mM, 1.1fold activation in presence of Na2S, 4.2fold in absence of Na2S
Rhodococcus globerulus
4.99.1.5
thioglycerol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 4.06fold
Rhodococcus globerulus
4.99.1.5
vitamin K3
activates
Rhodococcus globerulus
Application
EC Number
Application
Commentary
Organism
4.99.1.5
synthesis
may be a useful biocatalyst for the production of various nitriles from the corresponding aldoximes
Rhodococcus globerulus
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
4.99.1.5
oxd gene, overexpression in Escherichia coli JM109, sequencing, gene cluster organisation
Rhodococcus globerulus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.99.1.5
1,2,3-benzenetriol
slight inhibition
Rhodococcus globerulus
4.99.1.5
Cd2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
Co2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
Cu+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
Cu2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
dimethylphenylenediamine
slight inhibition
Rhodococcus globerulus
4.99.1.5
guaiacol
slight inhibition
Rhodococcus globerulus
4.99.1.5
heavy metal ion
1 mM
Rhodococcus globerulus
4.99.1.5
miconazole
slight inhibition
Rhodococcus globerulus
4.99.1.5
additional information
not inhibited by hydrazine, KCN, NaN3, and NH2OH
Rhodococcus globerulus
4.99.1.5
nitroblue tetrazolium
slight inhibition
Rhodococcus globerulus
4.99.1.5
p-phenylenediamine
slight inhibition
Rhodococcus globerulus
4.99.1.5
phenazine methosulfate
slight inhibition
Rhodococcus globerulus
4.99.1.5
phenylhydrazine
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
4.99.1.5
sulfhydryl reagent
1 mM
Rhodococcus globerulus
4.99.1.5
tetramethylphenylenediamine
slight inhibition
Rhodococcus globerulus
4.99.1.5
Tiron
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
4.99.1.5
trimethylhydroquinone
slight inhibition
Rhodococcus globerulus
4.99.1.5
Zn2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.99.1.5
additional information
-
additional information
Km for the activating Na2S is 0.0408 mM
Rhodococcus globerulus
4.99.1.5
1.13
-
(E/Z)-n-valeraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
1.13
-
(E/Z)-cyclohexanecarboxaldehyde oxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
1.4
-
(Z)-phenylacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
1.73
-
(E/Z)-n-butyraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
2.31
-
(Z)-3-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
2.94
-
(E/Z)-n-capronaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
3.23
-
(E/Z)-mandelaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
3.91
-
(E/Z)-indoleacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
3.97
-
(E/Z)-isovaleraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
5.13
-
(E/Z)-propionaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
5.54
-
(E/Z)-isobutyraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
6.76
-
(E/Z)-isocapronaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
11.9
-
(E/Z)-2-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
20
-
(E)-pyridine-3-aldoxime
pH 7, 30°C
Rhodococcus globerulus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.99.1.5
Cd2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Co2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Cu+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Cu2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Fe2+
heme iron is present in a reduced form, 1 mM, activates
Rhodococcus globerulus
4.99.1.5
Fe3+
1 mM, activates
Rhodococcus globerulus
4.99.1.5
Zn2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.99.1.5
39892
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
4.99.1.5
42000
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
4.99.1.5
76200
-
wild-type OxdRG, gel filtration
Rhodococcus globerulus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.99.1.5
alkylaldoxime
Rhodococcus globerulus
responsible for the metabolism of alkylaldoxime
alkylnitrile + H2O
-
Rhodococcus globerulus
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
4.99.1.5
Rhodococcus globerulus
Q76EV4
-
-
4.99.1.5
Rhodococcus globerulus A-4
Q76EV4
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
4.99.1.5
wild-type OxdRG: 104fold, recombinant OxdRG expressed in Escherichia coli JM109: 14.9fold
Rhodococcus globerulus
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.99.1.5
0.0879
-
pH 7, 30°C, wild-type OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
4.99.1.5
2.18
-
pH 7, 30°C, recombinant OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4.99.1.5
(E)-pyridine-3-aldoxime
not Z-form, poor substrate, 0.78% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
pyridine-3-nitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-2-phenylpropionaldoxime
5.23% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
2-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus
?
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus A-4
?
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
(E/Z)-indoleacetaldoxime
7.29% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
indoleacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-isobutyraldoxime
26.5% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isobutyronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-isocapronaldoxime
88.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isocaprononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-isovaleraldoxime
58.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isovaleronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-mandelaldoxime
3.09% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
mandelonitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
(E/Z)-n-capronaldoxime
64.2% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-caprononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
(E/Z)-propionaldoxime
7.76% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
propiononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(Z)-3-phenylpropionaldoxime
67.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
3-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus
nitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus A-4
nitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
alkylaldoxime
responsible for the metabolism of alkylaldoxime
650189
Rhodococcus globerulus
alkylnitrile + H2O
-
650189
Rhodococcus globerulus
?
Subunits
EC Number
Subunits
Commentary
Organism
4.99.1.5
dimer
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
Synonyms
EC Number
Synonyms
Commentary
Organism
4.99.1.5
alkylaldoxime dehydratase
-
Rhodococcus globerulus
4.99.1.5
OxdRG
-
Rhodococcus globerulus
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.99.1.5
30
-
around, 0.1 M potassium phosphate buffer, pH 8
Rhodococcus globerulus
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.99.1.5
40
-
30 min, 0.1 M potassium phosphate buffer, pH 8, 35% loss of activity
Rhodococcus globerulus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.99.1.5
8
-
0.1 M potassium phosphate buffer
Rhodococcus globerulus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
4.99.1.5
heme b
prosthetic group, protoheme IX, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
4.99.1.5
protoheme IX
prosthetic group, heme b, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.99.1.5
2-mercaptoethanol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.13fold
Rhodococcus globerulus
4.99.1.5
cysteamine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 3.93fold
Rhodococcus globerulus
4.99.1.5
duroquinone
activates
Rhodococcus globerulus
4.99.1.5
FAD
1 mM, 1.2fold activation in presence of Na2S, 4.5fold in absence of Na2S
Rhodococcus globerulus
4.99.1.5
FMN
1 mM, 1.7fold activation in presence of Na2S, 2.4fold in absence of Na2S
Rhodococcus globerulus
4.99.1.5
L-cysteine
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 2.39fold
Rhodococcus globerulus
4.99.1.5
additional information
not activated by NaHSO3, Na2SO4, NaHSO4 or Na2S2O7
Rhodococcus globerulus
4.99.1.5
Na2S
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.35fold
Rhodococcus globerulus
4.99.1.5
Na2S2O4
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 8.13fold
Rhodococcus globerulus
4.99.1.5
Na2S2O5
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 5.84fold
Rhodococcus globerulus
4.99.1.5
Na2SO3
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 7.51fold
Rhodococcus globerulus
4.99.1.5
reducing reagent
requirement, activates
Rhodococcus globerulus
4.99.1.5
riboflavin
1 mM, 1.1fold activation in presence of Na2S, 4.2fold in absence of Na2S
Rhodococcus globerulus
4.99.1.5
thioglycerol
activates, (Z)-phenylacetaldoxime dehydration, 1 mM, 4.06fold
Rhodococcus globerulus
4.99.1.5
vitamin K3
activates
Rhodococcus globerulus
Application (protein specific)
EC Number
Application
Commentary
Organism
4.99.1.5
synthesis
may be a useful biocatalyst for the production of various nitriles from the corresponding aldoximes
Rhodococcus globerulus
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.99.1.5
oxd gene, overexpression in Escherichia coli JM109, sequencing, gene cluster organisation
Rhodococcus globerulus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
4.99.1.5
heme b
prosthetic group, protoheme IX, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
4.99.1.5
protoheme IX
prosthetic group, heme b, heme iron is present in a reduced form, contains 0.37 mol heme per mol of enzyme
Rhodococcus globerulus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.99.1.5
1,2,3-benzenetriol
slight inhibition
Rhodococcus globerulus
4.99.1.5
Cd2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
Co2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
Cu+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
Cu2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
4.99.1.5
dimethylphenylenediamine
slight inhibition
Rhodococcus globerulus
4.99.1.5
guaiacol
slight inhibition
Rhodococcus globerulus
4.99.1.5
heavy metal ion
1 mM
Rhodococcus globerulus
4.99.1.5
miconazole
slight inhibition
Rhodococcus globerulus
4.99.1.5
additional information
not inhibited by hydrazine, KCN, NaN3, and NH2OH
Rhodococcus globerulus
4.99.1.5
nitroblue tetrazolium
slight inhibition
Rhodococcus globerulus
4.99.1.5
p-phenylenediamine
slight inhibition
Rhodococcus globerulus
4.99.1.5
phenazine methosulfate
slight inhibition
Rhodococcus globerulus
4.99.1.5
phenylhydrazine
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
4.99.1.5
sulfhydryl reagent
1 mM
Rhodococcus globerulus
4.99.1.5
tetramethylphenylenediamine
slight inhibition
Rhodococcus globerulus
4.99.1.5
Tiron
inhibits dehydration of (Z)-3-phenylpropionaldoxime, but not of (E/Z)-n-valeraldoxime
Rhodococcus globerulus
4.99.1.5
trimethylhydroquinone
slight inhibition
Rhodococcus globerulus
4.99.1.5
Zn2+
strong inhibition at 1 mM, activates at 0.1 mM
Rhodococcus globerulus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.99.1.5
additional information
-
additional information
Km for the activating Na2S is 0.0408 mM
Rhodococcus globerulus
4.99.1.5
1.13
-
(E/Z)-n-valeraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
1.13
-
(E/Z)-cyclohexanecarboxaldehyde oxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
1.4
-
(Z)-phenylacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
1.73
-
(E/Z)-n-butyraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
2.31
-
(Z)-3-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
2.94
-
(E/Z)-n-capronaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
3.23
-
(E/Z)-mandelaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
3.91
-
(E/Z)-indoleacetaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
3.97
-
(E/Z)-isovaleraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
5.13
-
(E/Z)-propionaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
5.54
-
(E/Z)-isobutyraldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
6.76
-
(E/Z)-isocapronaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
11.9
-
(E/Z)-2-phenylpropionaldoxime
pH 7, 30°C
Rhodococcus globerulus
4.99.1.5
20
-
(E)-pyridine-3-aldoxime
pH 7, 30°C
Rhodococcus globerulus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.99.1.5
Cd2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Co2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Cu+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Cu2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
4.99.1.5
Fe2+
heme iron is present in a reduced form, 1 mM, activates
Rhodococcus globerulus
4.99.1.5
Fe3+
1 mM, activates
Rhodococcus globerulus
4.99.1.5
Zn2+
activates at 0.1 mM, strong inhibition at 1 mM
Rhodococcus globerulus
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.99.1.5
39892
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
4.99.1.5
42000
-
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
4.99.1.5
76200
-
wild-type OxdRG, gel filtration
Rhodococcus globerulus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
4.99.1.5
alkylaldoxime
Rhodococcus globerulus
responsible for the metabolism of alkylaldoxime
alkylnitrile + H2O
-
Rhodococcus globerulus
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.99.1.5
wild-type OxdRG: 104fold, recombinant OxdRG expressed in Escherichia coli JM109: 14.9fold
Rhodococcus globerulus
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.99.1.5
0.0879
-
pH 7, 30°C, wild-type OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
4.99.1.5
2.18
-
pH 7, 30°C, recombinant OxdRG, (Z)-phenylacetaldoxime as substrate, in presence of 1 mM Na2S
Rhodococcus globerulus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4.99.1.5
(E)-pyridine-3-aldoxime
not Z-form, poor substrate, 0.78% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
pyridine-3-nitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-2-phenylpropionaldoxime
5.23% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
2-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus
?
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-cyclohexanecarboxaldehyde oxime
best substrate
650189
Rhodococcus globerulus A-4
?
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
(E/Z)-indoleacetaldoxime
7.29% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
indoleacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-isobutyraldoxime
26.5% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isobutyronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-isocapronaldoxime
88.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isocaprononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-isovaleraldoxime
58.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
isovaleronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-mandelaldoxime
3.09% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
mandelonitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-butyraldoxime
48.9% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-butyronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
(E/Z)-n-capronaldoxime
64.2% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-caprononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(E/Z)-n-valeraldoxime
39.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
n-valeronitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
(E/Z)-propionaldoxime
7.76% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
propiononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(Z)-3-phenylpropionaldoxime
67.3% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
3-phenylpropiononitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
(Z)-phenylacetaldoxime
26.6% of the activity with (E/Z)-cyclohexanecarboxaldehyde oxime
650189
Rhodococcus globerulus A-4
phenylacetonitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus
nitrile + H2O
-
650189
Rhodococcus globerulus
?
4.99.1.5
aldoxime
wide range of substrates, aliphatic aldoximes are more effective substrates than arylalkyl aldoximes, aromatic aldoximes are also dehydrated
650189
Rhodococcus globerulus A-4
nitrile + H2O
-
650189
Rhodococcus globerulus A-4
?
4.99.1.5
alkylaldoxime
responsible for the metabolism of alkylaldoxime
650189
Rhodococcus globerulus
alkylnitrile + H2O
-
650189
Rhodococcus globerulus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.99.1.5
dimer
2 * 42000, wild-type OxdRG, SDS-PAGE, 2 * 39892, calculated from the sequence
Rhodococcus globerulus
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
4.99.1.5
30
-
around, 0.1 M potassium phosphate buffer, pH 8
Rhodococcus globerulus
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4.99.1.5
40
-
30 min, 0.1 M potassium phosphate buffer, pH 8, 35% loss of activity
Rhodococcus globerulus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.99.1.5
8
-
0.1 M potassium phosphate buffer
Rhodococcus globerulus