EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.10 | 1-Phenyl-2-thiourea | competitive | Vibrio proteolyticus | |
3.4.11.10 | EDTA | - |
Vibrio proteolyticus | |
3.4.11.10 | L-leucine anilide | - |
Vibrio proteolyticus | |
3.4.11.10 | L-leucine anisidide | - |
Vibrio proteolyticus | |
3.4.11.10 | Phenylurea | - |
Vibrio proteolyticus | |
3.4.11.10 | thionoleucine-S-anilide | - |
Vibrio proteolyticus | |
3.4.11.10 | thionoleucine-S-anisidide | - |
Vibrio proteolyticus | |
3.4.11.10 | Urea | competitive | Vibrio proteolyticus | |
3.4.11.22 | 1-Phenyl-2-thiourea | competitive | Vibrio proteolyticus | |
3.4.11.22 | Urea | competitive | Vibrio proteolyticus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | additional information | - |
additional information | kinetics with Zn and/or Cd-containing enzyme, overview | Vibrio proteolyticus | |
3.4.11.10 | 4 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 4 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 4 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 4.6 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 5 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 7 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 7.8 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 8 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 10 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 11 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 15 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 32 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 34 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 45 | - |
L-leucine anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 50 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 61 | - |
L-leucine anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 78 | - |
L-leucine anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 4 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 4 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 4 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 4.6 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 5 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 7 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 7.8 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 8 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 10 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 11 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 15 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 32 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 34 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 45 | - |
L-leucin-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 50 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 61 | - |
L-leucine-anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 78 | - |
L-leucine-anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.10 | Cd2+ | the bimetallic enzyme contains either 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ | Vibrio proteolyticus | |
3.4.11.10 | Cd2+ | can substitute for one or both of the Zn2+ ions | Vibrio proteolyticus | |
3.4.11.10 | Co2+ | Co2+ can substitute for Cd2+ in the bimetallic enzyme | Vibrio proteolyticus | |
3.4.11.10 | Co2+ | can substitute for one or both of the Zn2+ ions | Vibrio proteolyticus | |
3.4.11.10 | additional information | bimetallic enzyme, binding conformations, overview | Vibrio proteolyticus | |
3.4.11.10 | Zn2+ | the bimetallic enzyme contains either 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ | Vibrio proteolyticus | |
3.4.11.10 | Zn2+ | zinc-metallopeptidase, 2 ions per enzyme molecule | Vibrio proteolyticus | |
3.4.11.22 | Cd2+ | the bimetallic enzyme contains eihter 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ | Vibrio proteolyticus | |
3.4.11.22 | Co2+ | Co2+ can substitute for Cd2+ in the bimetallic enzyme | Vibrio proteolyticus | |
3.4.11.22 | Zn2+ | the bimetallic enzyme contains eihter 2 Zn2+ or 2 Cd2+ or Zn2+ and Cd2+ | Vibrio proteolyticus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.10 | Vibrio proteolyticus | - |
- |
- |
3.4.11.22 | Vibrio proteolyticus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.10 | - |
Vibrio proteolyticus |
3.4.11.10 | native enzyme | Vibrio proteolyticus |
3.4.11.22 | - |
Vibrio proteolyticus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.11.10 | Release of an N-terminal amino acid, preferentially leucine, but not glutamic or aspartic acids. | substrate binding mechanism and structure | Vibrio proteolyticus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.10 | L-Leu-4-nitroanilide + H2O | - |
Vibrio proteolyticus | L-Leu + 4-nitroaniline | - |
? | |
3.4.11.10 | L-Leu-7-amido-4-methylcoumarin + H2O | - |
Vibrio proteolyticus | L-Leu + 7-amino-4-methylcoumarin | - |
? | |
3.4.11.10 | L-leucine 4-nitroanilide + H2O | - |
Vibrio proteolyticus | L-leucine + 4-nitroaniline | - |
? | |
3.4.11.10 | L-leucine anilide + H2O | - |
Vibrio proteolyticus | L-leucine + aniline | - |
? | |
3.4.11.10 | L-leucine-4-anisidide + H2O | - |
Vibrio proteolyticus | L-leucine + anisidine | - |
? | |
3.4.11.10 | thionoleucine-4-anisidide + H2O | - |
Vibrio proteolyticus | thionoleucine + anisidine | - |
? | |
3.4.11.10 | thionoleucine-S-anilide + H2O | - |
Vibrio proteolyticus | thionoleucine + aniline | - |
? | |
3.4.11.22 | L-leucine 4-nitroanilide + H2O | - |
Vibrio proteolyticus | L-leucine + 4-nitroaniline | - |
? | |
3.4.11.22 | L-leucine-4-anisidide + H2O | - |
Vibrio proteolyticus | L-leucine + anisidine | - |
? | |
3.4.11.22 | L-leucine-anilide + H2O | - |
Vibrio proteolyticus | L-leucine + aniline | - |
? | |
3.4.11.22 | thionoleucine-4-anisidide + H2O | - |
Vibrio proteolyticus | thionoleucine + anisidine | - |
? | |
3.4.11.22 | thionoleucine-S-anilide + H2O | - |
Vibrio proteolyticus | thionoleucine + aniline | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.10 | AAP | - |
Vibrio proteolyticus |
3.4.11.10 | Aeromonas proteolytica aminopeptidase | - |
Vibrio proteolyticus |
3.4.11.10 | More | see also EC 3.4.11.22 | Vibrio proteolyticus |
3.4.11.22 | AAP | - |
Vibrio proteolyticus |
3.4.11.22 | Aeromonas proteolytica aminopeptidase | - |
Vibrio proteolyticus |
3.4.11.22 | More | see also EC 3.4.11.10 | Vibrio proteolyticus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 25 | - |
assay at | Vibrio proteolyticus |
3.4.11.22 | 25 | - |
assay at | Vibrio proteolyticus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | 3 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 3.8 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 5.3 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 6.3 | - |
L-leucine anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 7.5 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 7.7 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 7.9 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 9.3 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 9.5 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 10.6 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 11.1 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 11.5 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 12.2 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 15.1 | - |
L-leucine anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 16 | - |
L-leucine anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 16.9 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 23.3 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.10 | 60 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 3 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 3.8 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 5.3 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 6.3 | - |
L-leucine-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 7.5 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 7.7 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 7.9 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 9.3 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 9.5 | - |
L-leucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 10.6 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 11.1 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 11.5 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 12.2 | - |
thionoleucine-4-anisidide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 15.1 | - |
L-leucine-anilide | pH 8.0, 25°C, enzyme with Cd2+ and Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 16 | - |
L-leucine-anilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 16.9 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 23.3 | - |
thionoleucine-S-anilide | pH 8.0, 25°C, enzyme with Zn2+ and Cd2+ bound | Vibrio proteolyticus | |
3.4.11.22 | 60 | - |
L-leucine 4-nitroanilide | pH 8.0, 25°C, enzyme with 2 Zn2+ bound | Vibrio proteolyticus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 8 | - |
assay at | Vibrio proteolyticus |
3.4.11.22 | 8 | - |
assay at | Vibrio proteolyticus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | additional information | - |
additional information | inhibition kinetics with Zn and/or Cd-containing enzyme, overview | Vibrio proteolyticus | |
3.4.11.10 | 0.24 | - |
1-Phenyl-2-thiourea | pH 8.0, 25°C | Vibrio proteolyticus | |
3.4.11.10 | 4.6 | - |
Urea | pH 8.0, 25°C | Vibrio proteolyticus | |
3.4.11.22 | 0.24 | - |
1-Phenyl-2-thiourea | pH 8.0, 25°C | Vibrio proteolyticus | |
3.4.11.22 | 4.6 | - |
Urea | pH 8.0, 25°C | Vibrio proteolyticus |