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Literature summary extracted from

  • Harpel, M.R.; Larimer, F.W.; Hartman, F.C.
    Multifaceted roles of Lys166 of ribulose-bisphosphate carboxylase/oxygenase as discerned by product analysis and chemical rescue of site-directed mutants (2002), Biochemistry, 41, 1390-1397.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.39 C58S site-directed mutagenesis, elimination of the chemically active site without any loss of activity Rhodospirillum rubrum
4.1.1.39 K166C site-directed mutagenesis, able to process RuBP, but the major product is DiMP derived from beta-elimination of phosphate from the enediol Rhodospirillum rubrum
4.1.1.39 K166C/C58S site-directed mutagenesis, virtually inactive double mutant Rhodospirillum rubrum
4.1.1.39 K166G site-directed mutagenesis, able to process RuBP, but the major product is DiMP derived from beta-elimination of phosphate from the enediol Rhodospirillum rubrum
4.1.1.39 K166S site-directed mutagenesis, able to process RuBP, but the major product is DiMP derived from beta-elimination of phosphate from the enediol Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O Rhodospirillum rubrum rate-limiting reaction of overall photosynthetic CO2 assimilation and photorespiratory carbon oxidation 3-phospho-D-glycerate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.39 Rhodospirillum rubrum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.39 mutant rbc genes transferred into pFL260 for expression in Escherichia coli MV1190 Rhodospirillum rubrum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O
-
 Rhodospirillum rubrum 3-phospho-D-glycerate
-
 ?
4.1.1.39 D-ribulose 1,5-bisphosphate + CO2 + H2O rate-limiting reaction of overall photosynthetic CO2 assimilation and photorespiratory carbon oxidation Rhodospirillum rubrum 3-phospho-D-glycerate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
4.1.1.39 ribulose-bisphosphate carboxylase/oxygenase
-
 Rhodospirillum rubrum
4.1.1.39 Rubisco
-
 Rhodospirillum rubrum