EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.1.17 | A98P | kcat is decreased 3400fold compared to wild type and KM is increased 13fold, mutant enzyme has a severely compromised ability for catalyzing the formation of (3R)-3-hydroxybutanoyl-CoA | Rattus norvegicus |
4.2.1.17 | E144D | 60fold decreases in kcat with little change in KM | Rattus norvegicus |
4.2.1.17 | E144Q | 3000fold decreases in kcat with little change in KM. The mutant is unable to catalyze the formation of (3R)-3-hydroxybutanoyl-CoA even when the incubation is extended to 4 days | Rattus norvegicus |
4.2.1.17 | E164D | 1200fold decreases in kcat with little change in KM. First-order rate constant for the formation of (3R)-3-hydroxybutanoyl-CoA is similar to wild-type value | Rattus norvegicus |
4.2.1.17 | E164Q | 340000fold decreases in kcat with little change in KM. While wild-type enoyl-CoA hydratase catalyzes the rapid interconversion of substrate and the (3S)-3-hydroxybutanoyl-CoA product relative to the rate of (3R)-3-hydroxybutanoyl-CoA formation, E164Q catalyzes the formation of both product enantiomers at similar rates | Rattus norvegicus |
4.2.1.17 | G141P | 1600000fold decrease in kcat with no change in KM, mutant enzyme has a severely compromised ability for catalyzing the formation of (3R)-3-hydroxybutanoyl-CoA | Rattus norvegicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.17 | 0.003 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme G141P | Rattus norvegicus | |
4.2.1.17 | 0.015 | - |
crotonyl-CoA | pH 7.4, 25°C, wild-type enzyme | Rattus norvegicus | |
4.2.1.17 | 0.025 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme E144Q | Rattus norvegicus | |
4.2.1.17 | 0.032 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme E164D | Rattus norvegicus | |
4.2.1.17 | 0.041 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme E164Q | Rattus norvegicus | |
4.2.1.17 | 0.118 | - |
3'-dephosphocrotonyl-CoA | pH 7.4, 25°C, mutant enzyme E144D | Rattus norvegicus | |
4.2.1.17 | 0.195 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme A98P | Rattus norvegicus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
4.2.1.17 | mitochondrion | - |
Rattus norvegicus | 5739 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.17 | Rattus norvegicus | - |
recombinant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.2.1.17 | recombinant, wild-type and mutant enzymes | Rattus norvegicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.17 | 3'-dephosphocrotonyl-CoA + H2O | - |
Rattus norvegicus | ? | - |
? | |
4.2.1.17 | crotonyl-CoA + H2O | i.e. (E)-2-butenoyl-CoA. Reaction is catalyzed with a stereospecificity of 1 in 400000. The enzyme catalyzes the rapid interconversion of substrate and the (3S)-3-hydroxybutanoyl-CoA product relative to the rate of (3R)-3-hydroxybutanoyl-CoA formation. Formation of the correct product enantiomer requires an intact oxyanion hole and optimal positioning of the substrate with respect to two catalytic glutamates (E144 and E164) in the active site | Rattus norvegicus | (3S)-3-hydroxybutanoyl-CoA | - |
r |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.17 | 25 | - |
assay at | Rattus norvegicus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.2.1.17 | 0.0011 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme G141P | Rattus norvegicus | |
4.2.1.17 | 0.0053 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme E164Q | Rattus norvegicus | |
4.2.1.17 | 0.53 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme A98P | Rattus norvegicus | |
4.2.1.17 | 0.6 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme E144Q | Rattus norvegicus | |
4.2.1.17 | 1.5 | - |
crotonyl-CoA | pH 7.4, 25°C, mutant enzyme E164D | Rattus norvegicus | |
4.2.1.17 | 26 | - |
3'-dephosphocrotonyl-CoA | pH 7.4, 25°C, mutant enzyme E144D | Rattus norvegicus | |
4.2.1.17 | 1790 | - |
crotonyl-CoA | pH 7.4, 25°C, wild-type enzyme | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.1.17 | 7.4 | - |
assay at | Rattus norvegicus |