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Literature summary extracted from
- In vitro conversion of propionate to pyruvate by Salmonella enterica enzymes: 2-methylcitrate dehydratase (PrpD) and aconitase Enzymes catalyze the conversion of 2-methylcitrate to 2-methylisocitrate (2001), Biochemistry, 40, 4703-4713.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.79 | gene prpD in the operon prpBCDE, DNA sequence determination nand analysis, overexpression as N-terminally His-tagged protein in Escherichia coli BL21(DE3) | Salmonella enterica |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.79 | additional information | no requirement for metal ions and reducing agents | Salmonella enterica |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.79 | 2-Hydroxybutane-1,2,3-tricarboxylate | Salmonella enterica | - |
(Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? |  |
| 4.2.1.79 | additional information | Salmonella enterica | the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.79 | Salmonella enterica | P74840 | prpBCDE operon; serovar Typhimurium LT2, gene prpD of the prpBCDE operon | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.79 | recombinant His-tagged enzyme from Escherichia coli BL21(DE3), to homogeneity | Salmonella enterica |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.79 | 0.2 | - |
purified recombinant His-tagged enzyme, substrate citrate | Salmonella enterica |
| 4.2.1.79 | 2.6 | - |
purified recombinant His-tagged enzyme, substrate cis-aconitate | Salmonella enterica |
| 4.2.1.79 | 2.8 | - |
purified recombinant His-tagged enzyme, substrate 2-methylcitrate | Salmonella enterica |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.79 | 2-Hydroxybutane-1,2,3-tricarboxylate | - |
Salmonella enterica | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | - |
? | |
| 4.2.1.79 | 2-Hydroxybutane-1,2,3-tricarboxylate | i.e. 2-methylcitrate | Salmonella enterica | (Z)-But-2-ene-1,2,3-tricarboxylate + H2O | i.e. 2-methyl-cis-aconitate | ? | |
| 4.2.1.79 | cis-aconitate | - |
Salmonella enterica | ? | - |
? | |
| 4.2.1.79 | citrate | low activity with | Salmonella enterica | ? | - |
? | |
| 4.2.1.79 | additional information | enzyme does not catalyze the hydration of 2-methyl-cis-aconitate to 2-methylisocitrate, isocitrate is no substrate | Salmonella enterica | ? | - |
? | |
| 4.2.1.79 | additional information | the enzyme and the aconitase perform the 2-methylcitric acid cycle, overview | Salmonella enterica | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.1.79 | PrpD | - |
Salmonella enterica |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.79 | 37 | - |
assay at | Salmonella enterica |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.79 | 8 | - |
assay at | Salmonella enterica |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.79 | additional information | the enzyme contains no iron-sulfur center | Salmonella enterica |
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