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Literature summary extracted from

  • Gross, J.W.; Hegeman, A.D.; Gerratana, B.; Frey, P.A.
    Dehydration is catalyzed by glutamate-136 and aspartic acid-135 active site residues in Escherichia coli dTDP-glucose 4,6-dehydratase (2001), Biochemistry, 40, 12497-12504.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.46 D135135N/E136Q the turnover number for dTDP-6-fluoro-6-deoxyglucose is 340fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 204fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 D135A the steady-state rate of conversion of dTDP-6-fluoro-6-deoxyglucose to dTDP-4-keto-6-deoxyglucose is identical to that of wild-type. The turnover number for dTDP-6-fluoro-6-deoxyglucose is 1.5fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 223fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 D135N the steady-state rate of conversion of dTDP-6-fluoro-6-deoxyglucose to dTDP-4-keto-6-deoxyglucose is identical to that of wild-type. The turnover number for dTDP-6-fluoro-6-deoxyglucose is 1.2fold higher than that of the wild-type enzyme, the turnover number for dTDP-glucose is 124fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 E136A the turnover number for dTDP-6-fluoro-6-deoxyglucose is 690fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 288fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 E136Q the turnover number for dTDP-6-fluoro-6-deoxyglucose is 69fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 67fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 E198Q the turnover number for dTDP-6-fluoro-6-deoxyglucose is 190fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 258fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 K199M the turnover number for dTDP-6-fluoro-6-deoxyglucose is 260fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 115fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 K199R the turnover number for dTDP-6-fluoro-6-deoxyglucose is 680fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 288fold lower than that of the wild-type enzyme Escherichia coli
4.2.1.46 Y301F the turnover number for dTDP-6-fluoro-6-deoxyglucose is 31fold lower than that of the wild-type enzyme, the turnover number for dTDP-glucose is 117fold lower than that of the wild-type enzyme Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.46 Escherichia coli P27830
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.46 dTDP-6-fluoro-6-deoxyglucose substrate undergoes fluoride ion elimination instead of dehydration Escherichia coli dTDP-4-keto-6-deoxyglucose + F-
-
 ?
4.2.1.46 dTDP-glucose the conversion takes place in three steps: dehydrogenation to dTDP-4-ketoglucose, dehydration to dTDP-4-ketoglucose-5,6-ene and rereduction of C6 to the methyl group. Asp135 and Glu136 are the acid and base catalysts, respectively of the dehydration step Escherichia coli dTDP-4-dehydro-6-deoxy-D-glucose + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.46 0.000064
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme E136A Escherichia coli
4.2.1.46 0.000065
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme K199R Escherichia coli
4.2.1.46 0.00013
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme D135N/E136Q Escherichia coli
4.2.1.46 0.00017
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme K199M Escherichia coli
4.2.1.46 0.00023
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme E198Q Escherichia coli
4.2.1.46 0.00064
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme E136Q Escherichia coli
4.2.1.46 0.0014
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme Y301F Escherichia coli
4.2.1.46 0.017
-
 dTDP-glucose mutant enzyme E136A Escherichia coli
4.2.1.46 0.017
-
 dTDP-glucose mutant enzyme K199R Escherichia coli
4.2.1.46 0.019
-
 dTDP-glucose mutant enzyme E198Q Escherichia coli
4.2.1.46 0.022
-
 dTDP-glucose mutant enzyme D135A Escherichia coli
4.2.1.46 0.024
-
 dTDP-glucose mutant enzyme D135N/E136Q Escherichia coli
4.2.1.46 0.029
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme D135A Escherichia coli
4.2.1.46 0.0395
-
 dTDP-glucose mutant enzyme D135N Escherichia coli
4.2.1.46 0.042
-
 dTDP-glucose mutant enzyme Y301F Escherichia coli
4.2.1.46 0.0425
-
 dTDP-glucose mutant enzyme K199M Escherichia coli
4.2.1.46 0.044
-
 dTDP-6-fluoro-6-deoxyglucose wild-type enzyme Escherichia coli
4.2.1.46 0.053
-
 dTDP-6-fluoro-6-deoxyglucose mutant enzyme D135N Escherichia coli
4.2.1.46 0.073
-
 dTDP-glucose mutant enzyme E136Q Escherichia coli
4.2.1.46 4.9
-
 dTDP-glucose wild-type enzyme Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
4.2.1.46 NAD+ tightly bound Escherichia coli