Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Holyoak, T.; Nowak, T.
    Structural investigation of the binding of nucleotide to phosphoenolpyruvate carboxykinase by NMR (2001), Biochemistry, 40, 11037-11047.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.32 expression in Escherichia coli BL-21 Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.32 additional information mechanism of inactivation of PEPCK by Cys-273 modification due to inhibition of a dynamic motion that may occur upon nucleotide binding Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.32 additional information
-
 additional information kinetic data Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.1.32 cytosol cytosolic isoform Rattus norvegicus 5829
-
4.1.1.32 mitochondrion mitochondrial isoform Gallus gallus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.32 Mg2+ binds to enzyme more weakly than Mn2+ Rattus norvegicus
4.1.1.32 Mn2+ binds to the active site, activates Gallus gallus
4.1.1.32 Mn2+ binds to the active site, activates Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.32 67000
-
 1 * 67000 Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.32 GTP + oxaloacetate Gallus gallus primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 GTP + oxaloacetate Rattus norvegicus primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis GDP + phosphoenolpyruvate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.32 Gallus gallus
-
-
-
4.1.1.32 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.32
-
 Gallus gallus
4.1.1.32 recombinant enzyme Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.1.32 liver
-
 Gallus gallus
-
4.1.1.32 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.32 GTP + oxaloacetate specifically requires GTP or ITP, structural data of GTP binding Gallus gallus GDP + phosphoenolpyruvate + CO2
-
 r
4.1.1.32 GTP + oxaloacetate specifically requires GTP or ITP, structural data of GTP binding Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 r
4.1.1.32 GTP + oxaloacetate primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis Gallus gallus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 GTP + oxaloacetate primary role is the formation of phosphoenolpyruvate in the first committed step of gluconeogenesis Rattus norvegicus GDP + phosphoenolpyruvate + CO2
-
 ?
4.1.1.32 ITP + oxaloacetate specifically requires GTP or ITP Gallus gallus IDP + phosphoenolpyruvate + CO2
-
 r
4.1.1.32 ITP + oxaloacetate specifically requires GTP or ITP Rattus norvegicus IDP + phosphoenolpyruvate + CO2
-
 r

Subunits

EC Number Subunits Comment Organism
4.1.1.32 monomer
-
 Rattus norvegicus
4.1.1.32 monomer 1 * 67000 Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.32 GTP/ITP: oxaloacetate carboxylase (transphosphorylating)
-
 Gallus gallus
4.1.1.32 GTP/ITP: oxaloacetate carboxylase (transphosphorylating)
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.32 25
-
 assay at Gallus gallus
4.1.1.32 25
-
 assay at Rattus norvegicus