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Literature summary extracted from

  • Joerger, A.C.; Gosse, C.; Fessner, W.D.; Schulz, G.E.
    Catalytic action of fuculose 1-phosphate aldolase (class II) as derived from structure-directed mutagenesis (2000), Biochemistry, 39, 6033-6041.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.17
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.17
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.17 0.5
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, Y113F mutant Escherichia coli
4.1.2.17 1.8
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, R212A mutant Escherichia coli
4.1.2.17 2
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, wild-type enzyme Escherichia coli
4.1.2.17 3
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214D mutant Escherichia coli
4.1.2.17 3
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E215A mutant Escherichia coli
4.1.2.17 4.4
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, Y209F mutant Escherichia coli
4.1.2.17 6
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214Q and T26A mutant Escherichia coli
4.1.2.17 7
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, K207A mutant Escherichia coli
4.1.2.17 8
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214A mutant Escherichia coli
4.1.2.17 11
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, F206W mutant Escherichia coli
4.1.2.17 22
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, F131A mutant Escherichia coli
4.1.2.17 74
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214L mutant Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.17 additional information Escherichia coli fucose metabolism ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.17 Escherichia coli P0AB87
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.17
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.17 L-fuculose 1-phosphate
-
 Escherichia coli glycerone phosphate + (S)-lactaldehyde
-
 ?
4.1.2.17 additional information fucose metabolism Escherichia coli ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.17 0.4
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, Y113F mutant Escherichia coli
4.1.2.17 0.6
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, F131A mutant Escherichia coli
4.1.2.17 1.3
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, Y209F mutant Escherichia coli
4.1.2.17 4
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, T26A mutant Escherichia coli
4.1.2.17 8
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, F206W mutant Escherichia coli
4.1.2.17 12
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, R212A mutant Escherichia coli
4.1.2.17 13
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214L mutant Escherichia coli
4.1.2.17 19
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, wild-type enzyme and E214A mutant Escherichia coli
4.1.2.17 20
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214D and K207A mutant Escherichia coli
4.1.2.17 21
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E214Q mutant Escherichia coli
4.1.2.17 22
-
 L-Fuculose 1-phosphate pH 7.5, 37°C, E215A mutant Escherichia coli