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Literature summary extracted from

  • Chen, L.; Roberts, M.F.
    Overexpression, purification, and analysis of complementation behavior of E. coli SuhB protein: Comparison with bacterial and archaeal inositol monophosphatases (2000), Biochemistry, 39, 4145-4153.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, can not complement effects of inositol monophosphatase deficiency Methanocaldococcus jannaschii
3.1.3.25 expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, can not complement effects of inositol monophosphatase deficiency Archaeoglobus fulgidus
3.1.3.25 expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, can not complement effects of inositol monophosphatase deficiency Thermotoga maritima
3.1.3.25 expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, complements effects of inositol monophosphatase deficiency, expressed in Escherichia coli BL21-(DE3)pLsyS Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.25 D87N complete inactivation, resulting enzyme is still able to complement inositol monophosphatase deficiency in Escherichia coli CG1307 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.25 Li+
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.25 0.064
-
 myo-inositol 1-phosphate pH 8.0, 37°C Escherichia coli
3.1.3.25 0.079
-
 L-inositol 1-phosphate pH 8.0, 37°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Co2+ partial activation Escherichia coli
3.1.3.25 Fe2+ very weak activation Escherichia coli
3.1.3.25 Li+ very potent inhibitor Escherichia coli
3.1.3.25 Mg2+ required, highest activity with 6 mM Mg2+ Escherichia coli
3.1.3.25 Mn2+ partial activation Escherichia coli
3.1.3.25 Ni2+ partial activation Escherichia coli
3.1.3.25 Zn2+ very weak activation Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.25 29200
-
 calculated from amino acid sequence Escherichia coli
3.1.3.25 30500
-
 gel filtration at low ionic strength Escherichia coli
3.1.3.25 32000
-
 SDS-PAGE Escherichia coli
3.1.3.25 39000
-
 gel filtration in the presence of 250 mM KCl Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 myo-inositol 1-phosphate + H2O Methanocaldococcus jannaschii
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Thermotoga maritima
-
 myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O Escherichia coli very low concentration of inositol compounds myo-inositol + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.25 Archaeoglobus fulgidus
-
-
-
3.1.3.25 Escherichia coli
-
-
-
3.1.3.25 Methanocaldococcus jannaschii
-
-
-
3.1.3.25 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 high yield purification of recombinant protein from Escherichia coli BL21-(DE3)pLsyS Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.25 3.3
-
 purified enzyme, pH 8.0, 37°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.25 2'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3.1.3.25 glycerol 2-phosphate + H2O
-
 Escherichia coli glycerol + phosphate
-
 ?
3.1.3.25 additional information no activity with 4-nitrophenyl phosphate, 5'-AMP, NAD+, glucose 1-phosphate and glucose 6-phosphate Escherichia coli ?
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O D- and L-isomer, 2.5-fold higher activity with D-isomer Escherichia coli myo-inositol + phosphate
-
 ?
3.1.3.25 myo-inositol 1-phosphate + H2O very low concentration of inositol compounds Escherichia coli myo-inositol + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 monomer SDS-PAGE and gel filtration Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 80
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.25 40 100
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.25 70
-
 10% of activity lost within 5 min Escherichia coli