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Literature summary extracted from

  • Onesti, S.; Desogus, G.; Brevet, A.; Chen, J.; Plateau, P.; Blanquet, S.; Brick, P.
    Structural studies of lysyl-tRNA synthetase: conformational changes induced by substrate binding (2000), Biochemistry, 39, 12853-12861.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.6 recombinant expression Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.6 unliganded enzyme and in complex with L-lysine, vapor diffusion hanging drop method, protein solution: 20 mg/ml protein, 20 mM Tris-HCl, pH 8.0, 10 mM 2-mercaptoethanol, 10 mM MgCl2, plus equal volume of reservoir solution: 0.1 M HEPES, pH 7.5, 46-50% saturated ammonium sulfate solution, 2-4% polyethylene glycol 400, 15-20% glycerol, 4-18°C, X-ray difraction structure determination at 2.7 A resolution, and analysis Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.6 58000
-
 2 * 58000, recombinant enzyme, crystal structure Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys Escherichia coli
-
 AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.6 Escherichia coli P0A8N3 class II enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.6 recombinant enzyme Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.6 ATP + L-lysine + tRNALys = AMP + diphosphate + L-lysyl-tRNALys active site structure and substrate binding Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.6 ATP + L-lysine + tRNALys
-
 Escherichia coli AMP + L-lysyl-tRNALys + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.6 dimer 2 * 58000, recombinant enzyme, crystal structure Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.1.1.6 L-Lysine-transfer RNA ligase
-
 Escherichia coli
6.1.1.6 Lysine translase
-
 Escherichia coli
6.1.1.6 Lysine--tRNA ligase
-
 Escherichia coli
6.1.1.6 Lysine-tRNA synthetase
-
 Escherichia coli
6.1.1.6 LysRS
-
 Escherichia coli
6.1.1.6 Lysyl-transfer ribonucleate synthetase
-
 Escherichia coli
6.1.1.6 Lysyl-transfer RNA synthetase
-
 Escherichia coli
6.1.1.6 Lysyl-tRNA synthetase
-
 Escherichia coli
6.1.1.6 Synthetase, lysyl-transfer ribonucleate
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.6 ATP
-
 Escherichia coli