Literature summary extracted from
Avarbock, D.; Avarbock, A.; Rubin, H.
Differential regulation of opposing RelMtb activities by the aminoacylation state of a tRNA ribosome mRNA elMtb complex (2000), Biochemistry, 39, 11640-11648.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.7.2 |
medicine |
RelMtb is a key factor in Mycobacterium tuberculosis pathogenesis by regulating th intracellular concentration of (p)ppGpp. |
Mycobacterium tuberculosis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.7.2 |
the pET22b expression system is used |
Mycobacterium tuberculosis |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.7.2 |
0.41 |
- |
ppGpp |
pH 8.0, 30°C, KM is increased 3fold in the presence of the complex containing tRNA, ribosomes, and mRNA |
Mycobacterium tuberculosis |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.7.2 |
Mn2+ |
required, the optimal concentration is a 2-3fold molar excess over the nucleotide substrate, the addition in excess of optimal concentration decreases the rate of hydrolysis |
Mycobacterium tuberculosis |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
3.1.7.2 |
ppGpp + H2O |
Mycobacterium tuberculosis |
i.e. guanosine-3',5'-bis(diphosphate) |
Gpp + diphosphate |
i.e. guanosine 5'-diphosphate |
r |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.7.2 |
Mycobacterium tuberculosis |
- |
- |
- |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
3.1.7.2 |
guanosine 3',5'-bis(diphosphate) + H2O = GDP + diphosphate |
RelMtb is a dual-function enzyme carrying out ATP: GTP/GDP/ITP 3-diphosphoryltransferase and (p)ppGpp 3-diphosphohydrolase reactions. The differential regulation of the opposing activities of RelMtb is dependent on the ratio of uncharged to charged tRNA and the association of enzyme with a complex containing tRNA, ribosomes, and mRNA, i.e. RAC |
Mycobacterium tuberculosis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.7.2 |
ppGpp + H2O |
i.e. guanosine-3',5'-bis(diphosphate) |
Mycobacterium tuberculosis |
Gpp + diphosphate |
i.e. guanosine 5'-diphosphate |
? |
|
3.1.7.2 |
ppGpp + H2O |
i.e. guanosine-3',5'-bis(diphosphate) |
Mycobacterium tuberculosis |
Gpp + diphosphate |
i.e. guanosine 5'-diphosphate |
r |
|
Turnover Number [1/s]
EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Comment |
Organism |
Structure |
---|
3.1.7.2 |
2.97 |
- |
ppGpp |
pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA |
Mycobacterium tuberculosis |
|
3.1.7.2 |
3 |
6 |
ppGpp |
pH 8.0, 30°C, kcat is reduced 2fold in the presence of the complex containing tRNA, ribosomes, and mRNA |
Mycobacterium tuberculosis |
|
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.7.2 |
8 |
8.2 |
- |
Mycobacterium tuberculosis |