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Literature summary extracted from

  • Thoden, J.B.; Huang, X.; Raushel, F.M.; Holden, H.M.
    The small subunit of carbamoyl phosphate synthetase: snapshots along the reaction pathway (1999), Biochemistry, 38, 16158-16166.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.5 C269S mutant is unable to hydrolyse glutamine, the mutant is proved to be an adequate mimic for studies of the Michaelis complex Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3- Escherichia coli the product carbamoyl phosphate is an essential precursor of arginine and pyrimidine biosynthesis 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.5 Escherichia coli
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.5.5 2 ATP + L-glutamine + hydrogencarbonate + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate steps of reaction pathway for glutamine hydrolysis, the Michalis complex, the tetrahedral intermediate and glutamyl-thioester intermediate models, stereochemistry of the overall glutaminase reaction Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.5 2 ATP + L-Gln + HCO3-
-
 Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + L-Gln + HCO3- the product carbamoyl phosphate is an essential precursor of arginine and pyrimidine biosynthesis Escherichia coli 2 ADP + phosphate + L-Glu + carbamoyl phosphate
-
 ?
6.3.5.5 2 ATP + NH4+ + HCO3-
-
 Escherichia coli 2 ADP + phosphate + carbamoyl phosphate
-
 ?