BRENDA - Enzyme Database

Role of His159 in yeast enolase catalysis

Vinarov, D.A.; Nowak, T.; Biochemistry 38, 12138-12149 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression of the recombinant H159A and H159G mutants in Escherichia coli
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
H159A
mutation has no effect on conformation or enzyme-ligand complex, but yields an inactive enzyme
Saccharomyces cerevisiae
4.2.1.11
H159G
inactive form
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46500
-
x * 46500, SDS-PAGE, H159A and H159G mutants; x * 46500, SDS-PAGE, wild type
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
649859
Saccharomyces cerevisiae
phosphoenolpyruvate
-
649859
Saccharomyces cerevisiae
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46500, SDS-PAGE, H159A and H159G mutants; x * 46500, SDS-PAGE, wild type
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression of the recombinant H159A and H159G mutants in Escherichia coli
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
H159A
mutation has no effect on conformation or enzyme-ligand complex, but yields an inactive enzyme
Saccharomyces cerevisiae
4.2.1.11
H159G
inactive form
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46500
-
x * 46500, SDS-PAGE, H159A and H159G mutants; x * 46500, SDS-PAGE, wild type
Saccharomyces cerevisiae
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
649859
Saccharomyces cerevisiae
phosphoenolpyruvate
-
649859
Saccharomyces cerevisiae
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
?
x * 46500, SDS-PAGE, H159A and H159G mutants; x * 46500, SDS-PAGE, wild type
Saccharomyces cerevisiae