EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.13.22 | overexpression of wild-type and mutants as maltose-binding-protein fusion proteins | Enterococcus sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.13.22 | C78S/C157S | site-directed mutagenesis, reduced activity | Enterococcus sp. |
3.4.13.22 | D123A | site-directed mutagenesis, inactive, nearly no remaining Zn2+ | Enterococcus sp. |
3.4.13.22 | D145A/E146A | site-directed mutagenesis, 6fold lowered kcat compared to wild-type, 11% reduced zinc content compared to wild-type | Enterococcus sp. |
3.4.13.22 | E181A | site-directed mutagenesis, inactive, 21.8% reduced zinc content compared to wild-type | Enterococcus sp. |
3.4.13.22 | H116A | site-directed mutagenesis, inactive, 92.1% reduced zinc content compared to wild-type | Enterococcus sp. |
3.4.13.22 | H116A/H184A | site-directed mutagenesis, inactive, 97.5% reduced zinc content compared to wild-type | Enterococcus sp. |
3.4.13.22 | H13A | site-directed mutagenesis, activity equivalent to wild-type, 8.3% reduced zinc content compared to wild-type | Enterococcus sp. |
3.4.13.22 | H149A | site-directed mutagenesis, equivalent to wild-type | Enterococcus sp. |
3.4.13.22 | H149A/H150A | site-directed mutagenesis, 11fold lowered kcat, 14.9% reduced zinc content compared to wild-type | Enterococcus sp. |
3.4.13.22 | H150A | site-directed mutagenesis, equivalent to wild-type | Enterococcus sp. |
3.4.13.22 | H184A | site-directed mutagenesis, inactive, 99.08% reduced zinc content compared to wild-type | Enterococcus sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.22 | 1.8 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant C78S/C157S | Enterococcus sp. | |
3.4.13.22 | 2.1 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant H13A | Enterococcus sp. | |
3.4.13.22 | 2.4 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant H149A/H150A | Enterococcus sp. | |
3.4.13.22 | 2.5 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant wild-type enzyme | Enterococcus sp. | |
3.4.13.22 | 3.7 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant D145A/E146A | Enterococcus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.13.22 | Zn2+ | zinc binding residues H184, Asp123 and H116 in the active site, zinc content varies in mutant enzymes | Enterococcus sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.13.22 | D-Ala-D-Ala + H2O | Enterococcus sp. | - |
D-Ala + D-Ala | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.13.22 | Enterococcus sp. | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.13.22 | D-Ala-D-Ala + H2O = 2 D-Ala | reaction mechanism | Enterococcus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.13.22 | D-Ala-D-Ala + H2O | - |
Enterococcus sp. | D-Ala + D-Ala | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.13.22 | D-Ala-D-Ala dipeptidase VanX | - |
Enterococcus sp. |
3.4.13.22 | M15.011 | Merops-ID | Enterococcus sp. |
3.4.13.22 | VanX | - |
Enterococcus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.13.22 | 37 | - |
assay at | Enterococcus sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.13.22 | 0.64 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant C78S/C157S | Enterococcus sp. | |
3.4.13.22 | 9.1 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant H149A/H150A | Enterococcus sp. | |
3.4.13.22 | 20.2 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant D145A/E146A | Enterococcus sp. | |
3.4.13.22 | 54.1 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant C78S/C157S | Enterococcus sp. | |
3.4.13.22 | 83 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant mutant H13A | Enterococcus sp. | |
3.4.13.22 | 125 | - |
D-Ala-D-Ala | pH 8.0, 37°C, recombinant wild-type enzyme | Enterococcus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.13.22 | 8 | - |
assay at | Enterococcus sp. |