show Biochemical and mass spectrometric characterization of soluble ecto-5'-nucleotidase from bull seminal plasma
Fini, C.; Talamo, F.; Cherri, S.; Coli, M.; Floridi, A.; Ferrara, L.; Scaloni, A.; Biochem. J. 372, 443-451 (2003)
Data extracted from this reference:
Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
alpha,beta-methylene-adenosine 5'-diphosphate
inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4
Bos taurus
3.1.3.5
ATP
inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4
Bos taurus
Application
EC Number
Application
Commentary
Organism
3.1.3.5
medicine
target for a number of therapeutic drugs since increased levels of the enzyme correlate with various disease states
Bos taurus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.03
-
dCMP
isoenzyme 5'-NT-3
Bos taurus
3.1.3.5
0.04
-
dCMP
isoenzyme 5'-NT-2
Bos taurus
3.1.3.5
0.05
-
dCMP
isoenzyme 5'-NT-1
Bos taurus
3.1.3.5
0.06
-
5'-AMP
isoenzyme 5'-NT-2 and 5'-NT-4
Bos taurus
3.1.3.5
0.06
-
5'-IMP
isoenzyme 5'-NT-3
Bos taurus
3.1.3.5
0.06
-
dCMP
isoenzyme 5'-NT-4
Bos taurus
3.1.3.5
0.07
-
5'-GMP
isoenzyme 5'-NT-4
Bos taurus
3.1.3.5
0.07
-
5'-IMP
isoenzyme 5'-NT-2 and 5'-NT-4
Bos taurus
3.1.3.5
0.09
-
5'-GMP
isoenzyme 5'-NT-2
Bos taurus
3.1.3.5
0.11
-
5'-AMP
isoenzyme 5'-NT-1 and 5'-NT-3
Bos taurus
3.1.3.5
0.15
-
5'-IMP
isoenzyme 5'-NT-1
Bos taurus
3.1.3.5
0.16
-
5'-GMP
isoenzyme 5'-NT-3
Bos taurus
3.1.3.5
0.22
-
5'-GMP
isoenzyme 5'-NT-1
Bos taurus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
membrane
glycosylphosphatidyl-inositol-anchored
Bos taurus
16020
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
66200
-
x * 66200, SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
additional information
Bos taurus
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
?
-
Bos taurus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.5
Bos taurus
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.5
glycoprotein
differential glycosylation, especially in the oligosaccharides linked to Asn403, an increase in sialylated glycans, together with a decrease in high-mannose structures is observed arising from 5'-NT-1 to 5'-NT-4
Bos taurus
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.5
-
Bos taurus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
seminal plasma
-
Bos taurus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
300
-
-
Bos taurus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
649751
Bos taurus
adenosine + phosphate
-
649751
Bos taurus
?
3.1.3.5
5'-GMP + H2O
-
649751
Bos taurus
guanosine + phosphate
-
649751
Bos taurus
?
3.1.3.5
5'-IMP + H2O
-
649751
Bos taurus
inosine + phosphate
-
649751
Bos taurus
?
3.1.3.5
dCMP + H2O
-
649751
Bos taurus
deoxycytidine + phosphate
-
649751
Bos taurus
?
3.1.3.5
additional information
significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site
649751
Bos taurus
?
-
649751
Bos taurus
?
3.1.3.5
additional information
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
649751
Bos taurus
?
-
649751
Bos taurus
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.3.5
?
x * 66200, SDS-PAGE
Bos taurus
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.3.5
Bos taurus
isoenzyme 5'-NT-4
-
6.1
3.1.3.5
Bos taurus
isoenzyme 5'-NT-3
-
6.2
3.1.3.5
Bos taurus
isoenzyme 5'-NT-2
6.8
6.3
3.1.3.5
Bos taurus
isoenzyme 5'-NT-1
-
6.5
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.3.5
alpha,beta-methylene-adenosine 5'-diphosphate
inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4
Bos taurus
3.1.3.5
ATP
inhibits isoforms: 5'-NT-1, 5'-NT-2, 5'-NT-3, 5'-NT-4
Bos taurus
Application (protein specific)
EC Number
Application
Commentary
Organism
3.1.3.5
medicine
target for a number of therapeutic drugs since increased levels of the enzyme correlate with various disease states
Bos taurus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.5
0.03
-
dCMP
isoenzyme 5'-NT-3
Bos taurus
3.1.3.5
0.04
-
dCMP
isoenzyme 5'-NT-2
Bos taurus
3.1.3.5
0.05
-
dCMP
isoenzyme 5'-NT-1
Bos taurus
3.1.3.5
0.06
-
5'-AMP
isoenzyme 5'-NT-2 and 5'-NT-4
Bos taurus
3.1.3.5
0.06
-
5'-IMP
isoenzyme 5'-NT-3
Bos taurus
3.1.3.5
0.06
-
dCMP
isoenzyme 5'-NT-4
Bos taurus
3.1.3.5
0.07
-
5'-GMP
isoenzyme 5'-NT-4
Bos taurus
3.1.3.5
0.07
-
5'-IMP
isoenzyme 5'-NT-2 and 5'-NT-4
Bos taurus
3.1.3.5
0.09
-
5'-GMP
isoenzyme 5'-NT-2
Bos taurus
3.1.3.5
0.11
-
5'-AMP
isoenzyme 5'-NT-1 and 5'-NT-3
Bos taurus
3.1.3.5
0.15
-
5'-IMP
isoenzyme 5'-NT-1
Bos taurus
3.1.3.5
0.16
-
5'-GMP
isoenzyme 5'-NT-3
Bos taurus
3.1.3.5
0.22
-
5'-GMP
isoenzyme 5'-NT-1
Bos taurus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.5
membrane
glycosylphosphatidyl-inositol-anchored
Bos taurus
16020
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.3.5
66200
-
x * 66200, SDS-PAGE
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.5
additional information
Bos taurus
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
?
-
Bos taurus
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.3.5
glycoprotein
differential glycosylation, especially in the oligosaccharides linked to Asn403, an increase in sialylated glycans, together with a decrease in high-mannose structures is observed arising from 5'-NT-1 to 5'-NT-4
Bos taurus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.5
-
Bos taurus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.3.5
seminal plasma
-
Bos taurus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.5
300
-
-
Bos taurus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.5
5'-AMP + H2O
-
649751
Bos taurus
adenosine + phosphate
-
649751
Bos taurus
?
3.1.3.5
5'-GMP + H2O
-
649751
Bos taurus
guanosine + phosphate
-
649751
Bos taurus
?
3.1.3.5
5'-IMP + H2O
-
649751
Bos taurus
inosine + phosphate
-
649751
Bos taurus
?
3.1.3.5
dCMP + H2O
-
649751
Bos taurus
deoxycytidine + phosphate
-
649751
Bos taurus
?
3.1.3.5
additional information
significant differences in substrate specificity between the isoforms are interpreted in terms of variable glycosylation causing steric hindrance of the substrate-binding site
649751
Bos taurus
?
-
649751
Bos taurus
?
3.1.3.5
additional information
the soluble forms of enzyme in seminal plasma have a putative role in signalling interactions with spermatozoa following ejaculation and capacitations in the female reproductive tract
649751
Bos taurus
?
-
649751
Bos taurus
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.3.5
?
x * 66200, SDS-PAGE
Bos taurus
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.3.5
Bos taurus
isoenzyme 5'-NT-4
-
6.1
3.1.3.5
Bos taurus
isoenzyme 5'-NT-3
-
6.2
3.1.3.5
Bos taurus
isoenzyme 5'-NT-2
6.8
6.3
3.1.3.5
Bos taurus
isoenzyme 5'-NT-1
-
6.5