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Literature summary extracted from

  • Brown, I.E.; Mallen, M.H.; Charnock, S.J.; Davies, G.J.; Black, G.W.
    Pectate lyase 10A from Pseudomonas cellulosa is a modular enzyme containing a family 2a carbohydrate-binding module (2001), Biochem. J., 355, 155-165.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.2 expression in Escherichia coli Cellvibrio japonicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information Km-values: 0.104 mg/ml for polygalacturonic acid, 0.131 mg/ml for esterified citrus pectin, 1.837 mg/ml for 67% esterified citrus pectin, 1.837 mg/ml for 89% esterified citrus pectin Cellvibrio japonicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 Ca2+ optimal activity in presence of 2.0-4.0 mM Ca2+. No activity when Ca2+ is replaced with Co2+, Cu2+, Ba2+, Mg2+, Mn2+, Ni2+, Sr2+ or Zn2+ Cellvibrio japonicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 38000
-
 x * 38000, N-terminally His-tagged Pel10Acm, SDS-PAGE Cellvibrio japonicus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Cellvibrio japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2 38000 Da N-terminally His-tagged Pel10Acm Cellvibrio japonicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 67% esterified citrus pectin
-
 Cellvibrio japonicus ?
-
 ?
4.2.2.2 89% esterified citrus pectin
-
 Cellvibrio japonicus ?
-
 ?
4.2.2.2 pectin esterified citrus pectin Cellvibrio japonicus unsaturated oligogalacturonides
-
 ?
4.2.2.2 polygalacturonic acid i.e. pectate, endo-cleavage, the enzyme has a preference for sequences of non-esterified galacturonic acid residues Cellvibrio japonicus unsaturated oligogalacturonides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 38000, N-terminally His-tagged Pel10Acm, SDS-PAGE Cellvibrio japonicus

Synonyms

EC Number Synonyms Comment Organism
4.2.2.2 pectate lyase 10A
-
 Cellvibrio japonicus
4.2.2.2 Pel10A
-
 Cellvibrio japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 62
-
-
 Cellvibrio japonicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 47
-
 20 min, stable Cellvibrio japonicus
4.2.2.2 52
-
 20 min, 38% loss of activity Cellvibrio japonicus
4.2.2.2 57
-
 20 min, complete inactivation Cellvibrio japonicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.2.2 22
-
 89% esterified citrus pectin 37°C Cellvibrio japonicus
4.2.2.2 259.5
-
 polygalacturonic acid 37°C Cellvibrio japonicus
4.2.2.2 260
-
 polygalacturonic acid 37°C Cellvibrio japonicus
4.2.2.2 265
-
 67% esterified citrus pectin 37°C Cellvibrio japonicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 10
-
 polygalacturonic acid and esterified citrus pectin Cellvibrio japonicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.2.2 9.5 11 pH 9.5: about 25% of maximal activity, pH 11.0: about 35% of maximal activity with polygalacturonic acid as substrate Cellvibrio japonicus