BRENDA - Enzyme Database

Biochemical and spectroscopic characterization of Escherichia coli aconitases (AcnA and AcnB)

Jordan, P.A.; Tang, Y.; Bradbury, A.J.; Thomson, A.J.; Guest, J.R.; Biochem. J. 344, 739-746 (1999)
No PubMed abstract available

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
additional information
-
additional information
two values are obtained for KM-value with isocitrate as substrate, depending on substrate concentration
Escherichia coli
4.2.1.3
0.016
-
cis-aconitate
20C, pH 7.4, enzyme form AcnB
Escherichia coli
4.2.1.3
0.058
-
cis-aconitate
20C, pH 7.4, enzyme form AcnA
Escherichia coli
4.2.1.3
1.16
-
citrate
20C, pH 7.4, enzyme form AcnA
Escherichia coli
4.2.1.3
11
-
citrate
20C, pH 7.4, enzyme form AcnB
Escherichia coli
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
a significant proportion of the enzyme is in the inactive [3Fe-4S]1+ or apoenzyme forms. AcnB contains a much higher proportion of inactive enzyme than AcnA
Escherichia coli
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.3
Escherichia coli
-
and strain JRG3171, contains two major aconitases, AcnA and AcnB
-
4.2.1.3
Escherichia coli JRG2387
-
and strain JRG3171, contains two major aconitases, AcnA and AcnB
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.3
AcnA and AcnB
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate + H2O
-
649675
Escherichia coli
?
-
649675
Escherichia coli
?
4.2.1.3
cis-aconitate + H2O
-
649675
Escherichia coli JRG2387
?
-
649675
Escherichia coli JRG2387
?
4.2.1.3
citrate
-
649675
Escherichia coli
cis-aconitate + H2O
-
649675
Escherichia coli
?
4.2.1.3
citrate
-
649675
Escherichia coli JRG2387
cis-aconitate + H2O
-
649675
Escherichia coli JRG2387
?
4.2.1.3
isocitrate
-
649675
Escherichia coli
cis-aconitate + H2O
-
649675
Escherichia coli
?
4.2.1.3
isocitrate
-
649675
Escherichia coli JRG2387
cis-aconitate + H2O
-
649675
Escherichia coli JRG2387
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
-
enzyme form AcnA and AcnB, the maximal activity of AcnB is nearly 2fold the activity of AcnA
Escherichia coli
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
5
10
pH 5.0: about 75% of maximal activity, pH 10.0: about 60% of maximal activity, AcnA
Escherichia coli
4.2.1.3
6
9
pH 6.0: about 55% of maximal activity, pH 9.0: about 20% of maximal activity, AcnB
Escherichia coli
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.3
additional information
-
additional information
two values are obtained for KM-value with isocitrate as substrate, depending on substrate concentration
Escherichia coli
4.2.1.3
0.016
-
cis-aconitate
20C, pH 7.4, enzyme form AcnB
Escherichia coli
4.2.1.3
0.058
-
cis-aconitate
20C, pH 7.4, enzyme form AcnA
Escherichia coli
4.2.1.3
1.16
-
citrate
20C, pH 7.4, enzyme form AcnA
Escherichia coli
4.2.1.3
11
-
citrate
20C, pH 7.4, enzyme form AcnB
Escherichia coli
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.3
Iron
a significant proportion of the enzyme is in the inactive [3Fe-4S]1+ or apoenzyme forms. AcnB contains a much higher proportion of inactive enzyme than AcnA
Escherichia coli
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.3
AcnA and AcnB
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.3
cis-aconitate + H2O
-
649675
Escherichia coli
?
-
649675
Escherichia coli
?
4.2.1.3
cis-aconitate + H2O
-
649675
Escherichia coli JRG2387
?
-
649675
Escherichia coli JRG2387
?
4.2.1.3
citrate
-
649675
Escherichia coli
cis-aconitate + H2O
-
649675
Escherichia coli
?
4.2.1.3
citrate
-
649675
Escherichia coli JRG2387
cis-aconitate + H2O
-
649675
Escherichia coli JRG2387
?
4.2.1.3
isocitrate
-
649675
Escherichia coli
cis-aconitate + H2O
-
649675
Escherichia coli
?
4.2.1.3
isocitrate
-
649675
Escherichia coli JRG2387
cis-aconitate + H2O
-
649675
Escherichia coli JRG2387
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.3
7.5
-
enzyme form AcnA and AcnB, the maximal activity of AcnB is nearly 2fold the activity of AcnA
Escherichia coli
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.3
5
10
pH 5.0: about 75% of maximal activity, pH 10.0: about 60% of maximal activity, AcnA
Escherichia coli
4.2.1.3
6
9
pH 6.0: about 55% of maximal activity, pH 9.0: about 20% of maximal activity, AcnB
Escherichia coli