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Literature summary extracted from

  • Rodova, M.; Ankilova, V.; Safro, M.G.
    Human phenylalanyl-tRNA synthetase: cloning, characterization of the deduced amino acid sequences in terms of the structural domains and coordinately regulated expression of the alpha and beta subunits in chronic myeloid leukemia cells (1999), Biochem. Biophys. Res. Commun., 255, 765-773.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.20 DNA and amino acid sequence determination of the alpha- and beta-subunit and analysis, expression in COS-7 cells Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.20 55000
-
 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta, amino acid determination Homo sapiens
6.1.1.20 55000
-
 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta2, amino acid determination Homo sapiens
6.1.1.20 57000
-
 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta, amino acid determination Homo sapiens
6.1.1.20 57000
-
 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta2, amino acid determination Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe Homo sapiens
-
 AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 additional information Homo sapiens the catalytic function resides in the alpha-subunit, while the beta-subunit provides several binding-like domains for OB, RNP, SH3, and DNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.20 Homo sapiens Q9NSD9 class II enzyme
-
6.1.1.20 Homo sapiens Q9Y285 class II enzyme
-

Reaction

EC Number Reaction Comment Organism Reaction ID
6.1.1.20 ATP + L-phenylalanine + tRNAPhe = AMP + diphosphate + L-phenylalanyl-tRNAPhe tRNA recognition and binding mode Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.20 A-549 cell lung carcinoma cell line Homo sapiens
-
6.1.1.20 brain
-
 Homo sapiens
-
6.1.1.20 embryo
-
 Homo sapiens
-
6.1.1.20 G-361 cell
-
 Homo sapiens
-
6.1.1.20 heart
-
 Homo sapiens
-
6.1.1.20 HeLa-S3 cell high content Homo sapiens
-
6.1.1.20 HL-60 cell promyelocytic leukemia cell line, high content Homo sapiens
-
6.1.1.20 K-562 cell chronic myelogenous leukemic cell line, high content Homo sapiens
-
6.1.1.20 kidney low content, embryonic and adult Homo sapiens
-
6.1.1.20 liver low content Homo sapiens
-
6.1.1.20 lung low content Homo sapiens
-
6.1.1.20 MOLT-4 cell lymphoblastic leukemia cell line Homo sapiens
-
6.1.1.20 additional information enzyme content is increased in cancer cell lines, with the beta-subunit showing higher expression levels than the alpha-subunit Homo sapiens
-
6.1.1.20 pancreas
-
 Homo sapiens
-
6.1.1.20 placenta low content Homo sapiens
-
6.1.1.20 RAJI cell
-
 Homo sapiens
-
6.1.1.20 skeletal muscle
-
 Homo sapiens
-
6.1.1.20 SW-480 cell colorectal adenocarcinoma cell line, high content Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.20 ATP + L-phenylalanine + tRNAPhe
-
 Homo sapiens AMP + diphosphate + L-phenylalanyl-tRNAPhe
-
 ?
6.1.1.20 additional information the catalytic function resides in the alpha-subunit, while the beta-subunit provides several binding-like domains for OB, RNP, SH3, and DNA Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.1.1.20 dimer 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta, amino acid determination Homo sapiens
6.1.1.20 dimer 1 * 55000, alpha-subunit, + 1 * 57000, beta-subunit, alphabeta2, amino acid determination Homo sapiens
6.1.1.20 More the catalytic function resides in the alpha-subunit, while the beta-subunit provides several binding-like domains for OB, RNP, SH3, and DNA Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
6.1.1.20 CML33
-
 Homo sapiens
6.1.1.20 FRS
-
 Homo sapiens
6.1.1.20 HSPC173
-
 Homo sapiens
6.1.1.20 L-Phenylalanyl-tRNA synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanine translase
-
 Homo sapiens
6.1.1.20 Phenylalanine--tRNA ligase
-
 Homo sapiens
6.1.1.20 Phenylalanine-tRNA synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl transfer ribonucleic acid synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-transfer ribonucleate synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-transfer RNA ligase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-transfer RNA synthetase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-tRNA ligase
-
 Homo sapiens
6.1.1.20 Phenylalanyl-tRNA synthetase
-
 Homo sapiens
6.1.1.20 PheRS
-
 Homo sapiens
6.1.1.20 Synthetase, phenylalanyl-transfer ribonucleate
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.20 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.20 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
6.1.1.20 ATP
-
 Homo sapiens