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Literature summary extracted from
- Chorismate mutase of Thermus thermophilus is a monofunctional AroH class enzyme inhibited by tyrosine (2004), Arch. Microbiol., 181, 195-203.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | expression in Escherichia coli with a His-tag | Thermus thermophilus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | tyrosine | competitive inhibition, 0.5 M tyrosine leads to 40% inhibition | Thermus thermophilus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.29 | - |
chorismate | 70°C, pH 7.6 | Thermus thermophilus | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 15800 | - |
alpha3, 3 * 15800, SDS-PAGE | Thermus thermophilus |
| 5.4.99.5 | 47590 | - |
gel filtration | Thermus thermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | Thermus thermophilus | biosynthesis of aromatic amino acids | Prephenate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Thermus thermophilus | Q84FH6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | - |
Thermus thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 25 | - |
after affinity chromatography | Thermus thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Thermus thermophilus | Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | biosynthesis of aromatic amino acids | Thermus thermophilus | Prephenate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | trimer | alpha3, 3 * 15800, SDS-PAGE | Thermus thermophilus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | chorismate mutase | - |
Thermus thermophilus |
| 5.4.99.5 | TtCM | - |
Thermus thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 30 | - |
half-life of 61.7 min | Thermus thermophilus |
| 5.4.99.5 | 50 | - |
half-life of 42.7 min | Thermus thermophilus |
| 5.4.99.5 | 70 | - |
half-life of 8.1 min | Thermus thermophilus |
| 5.4.99.5 | 80 | - |
half-life of 6.5 min | Thermus thermophilus |
| 5.4.99.5 | 100 | - |
half-life of 1.4 min | Thermus thermophilus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 52 | - |
chorismate | 70°C, pH 7.6 | Thermus thermophilus | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.034 | - |
tyrosine | 70°C, pH 7.6 | Thermus thermophilus | |
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Release 2023.1 (January 2023)
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