BRENDA - Enzyme Database

Purification and characterization of homodimeric methylmalonyl-CoA mutase from Sinorhizobium meliloti

Miyamoto, E.; Watanabe, F.; Charles, T.C.; Yamaji, R.; Inui, H.; Nakano, Y.; Arch. Microbiol. 180, 151-154 (2003)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.2
5,5'-dithiobis(2-nitrobenzoic acid)
-
Sinorhizobium meliloti
5.4.99.2
Li+
slight inhibition at 3 mM
Sinorhizobium meliloti
5.4.99.2
Na+
slight inhibition at 3 mM
Sinorhizobium meliloti
5.4.99.2
p-chloromercuribenzoate
-
Sinorhizobium meliloti
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.2
0.00064
-
adenosylcobalamin
37°C, pH 7, in presence of 3 mM NH4+
Sinorhizobium meliloti
5.4.99.2
0.0086
-
adenosylcobalamin
37°C, pH 7, without NH4+
Sinorhizobium meliloti
5.4.99.2
0.11
-
(R,S)-methylmalonyl-CoA
37°C, pH 7, in presence of 3 mM NH4+
Sinorhizobium meliloti
5.4.99.2
0.13
-
(R,S)-methylmalonyl-CoA
37°C, pH 7, without NH4+
Sinorhizobium meliloti
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.99.2
NH4+
stimulates at 3 mM
Sinorhizobium meliloti
5.4.99.2
Rb+
stimulates at 3 mM
Sinorhizobium meliloti
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.99.2
80000
-
alpha2, 2 * 80000, SDS-PAGE
Sinorhizobium meliloti
5.4.99.2
165000
-
gel filtration
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5.4.99.2
(R)-2-methylmalonyl-CoA
Sinorhizobium meliloti
-
succinyl-CoA
-
Sinorhizobium meliloti
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
5.4.99.2
Sinorhizobium meliloti
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
5.4.99.2
to homogeneity
Sinorhizobium meliloti
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.99.2
10.85
-
after UNO Q-1 purification
Sinorhizobium meliloti
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
5.4.99.2
(R)-2-methylmalonyl-CoA
-
649513
Sinorhizobium meliloti
succinyl-CoA
-
649513
Sinorhizobium meliloti
?
5.4.99.2
methylmalonyl-CoA
-
649513
Sinorhizobium meliloti
succinyl-CoA
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.4.99.2
homodimer
alpha2, 2 * 80000, SDS-PAGE
Sinorhizobium meliloti
Synonyms
EC Number
Synonyms
Commentary
Organism
5.4.99.2
MCM
-
Sinorhizobium meliloti
5.4.99.2
methylmalonyl-CoA mutase
-
Sinorhizobium meliloti
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.4.99.2
37
-
-
Sinorhizobium meliloti
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.2
7
-
-
Sinorhizobium meliloti
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.2
adenosylcobalamin
-
Sinorhizobium meliloti
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.99.2
adenosylcobalamin
-
Sinorhizobium meliloti
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.4.99.2
5,5'-dithiobis(2-nitrobenzoic acid)
-
Sinorhizobium meliloti
5.4.99.2
Li+
slight inhibition at 3 mM
Sinorhizobium meliloti
5.4.99.2
Na+
slight inhibition at 3 mM
Sinorhizobium meliloti
5.4.99.2
p-chloromercuribenzoate
-
Sinorhizobium meliloti
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.4.99.2
0.00064
-
adenosylcobalamin
37°C, pH 7, in presence of 3 mM NH4+
Sinorhizobium meliloti
5.4.99.2
0.0086
-
adenosylcobalamin
37°C, pH 7, without NH4+
Sinorhizobium meliloti
5.4.99.2
0.11
-
(R,S)-methylmalonyl-CoA
37°C, pH 7, in presence of 3 mM NH4+
Sinorhizobium meliloti
5.4.99.2
0.13
-
(R,S)-methylmalonyl-CoA
37°C, pH 7, without NH4+
Sinorhizobium meliloti
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
5.4.99.2
NH4+
stimulates at 3 mM
Sinorhizobium meliloti
5.4.99.2
Rb+
stimulates at 3 mM
Sinorhizobium meliloti
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.4.99.2
80000
-
alpha2, 2 * 80000, SDS-PAGE
Sinorhizobium meliloti
5.4.99.2
165000
-
gel filtration
Sinorhizobium meliloti
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
5.4.99.2
(R)-2-methylmalonyl-CoA
Sinorhizobium meliloti
-
succinyl-CoA
-
Sinorhizobium meliloti
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.99.2
to homogeneity
Sinorhizobium meliloti
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.4.99.2
10.85
-
after UNO Q-1 purification
Sinorhizobium meliloti
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
5.4.99.2
(R)-2-methylmalonyl-CoA
-
649513
Sinorhizobium meliloti
succinyl-CoA
-
649513
Sinorhizobium meliloti
?
5.4.99.2
methylmalonyl-CoA
-
649513
Sinorhizobium meliloti
succinyl-CoA
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.4.99.2
homodimer
alpha2, 2 * 80000, SDS-PAGE
Sinorhizobium meliloti
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
5.4.99.2
37
-
-
Sinorhizobium meliloti
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4.99.2
7
-
-
Sinorhizobium meliloti