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Literature summary extracted from

  • Leaphart, A.B.; Trent Spencer, H.; Lovell, C.R.
    Site-directed mutagenesis of a potential catalytic and formyl phosphate binding site and substrate inhibition of N10-formyltetrahydrofolate synthetase (2002), Arch. Biochem. Biophys., 408, 137-143.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.3
-
 Moorella thermoacetica

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.3 R97E no activity Moorella thermoacetica
6.3.4.3 R97S 35% of wild-type kcat Moorella thermoacetica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.3 0.087
-
 ATP 27°C, pH 7.0, wild-type FTHFS Moorella thermoacetica
6.3.4.3 0.16
-
 ATP 27°C, pH 7.0, R97S mutant FTHFS Moorella thermoacetica
6.3.4.3 0.46
-
 formate 27°C, pH 7.0, wild-type FTHFS Moorella thermoacetica
6.3.4.3 0.95
-
 formate 27°C, pH 7.0, R97S mutant FTHFS Moorella thermoacetica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.3 ATP + formate + tetrahydrofolate Moorella thermoacetica
-
 ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.3 Moorella thermoacetica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.3 recombinant FTHFS Moorella thermoacetica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.3 ADP + carbamoylphosphate + tetrahydrofolate
-
 Moorella thermoacetica ATP + ?
-
 ?
6.3.4.3 ATP + formate + tetrahydrofolate
-
 Moorella thermoacetica ADP + phosphate + 10-formyltetrahydrofolate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.4.3 FTHFS
-
 Moorella thermoacetica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.3 0.39
-
 ATP 27°C, pH 7.0, R97S mutant FTHFS Moorella thermoacetica
6.3.4.3 0.4
-
 formate 27°C, pH 7.0, R97S mutant FTHFS Moorella thermoacetica
6.3.4.3 0.99
-
 ATP 27°C, pH 7.0, wild-type FTHFS Moorella thermoacetica
6.3.4.3 1.13
-
 formate 27°C, pH 7.0, wild-type FTHFS Moorella thermoacetica
6.3.4.3 6.08
-
 ATP 27°C, pH 7.0, wild-type FTHFS Moorella thermoacetica
6.3.4.3 6.08
-
 formate 27°C, pH 7.0, wild-type FTHFS Moorella thermoacetica