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Literature summary extracted from

  • Skipsey, M.; Andrews, C.J.; Townson, J.K.; Jepson, I.; Edwards, R.
    Cloning and characterization of glyoxalase I from soybean (2000), Arch. Biochem. Biophys., 374, 261-268.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expression of cDNA in Escherichia coli Glycine max

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.025
-
 glutathione-methylglyoxal 30°C, pH 7.5 Glycine max
4.4.1.5 0.027
-
 homoglutathione-phenylglyoxal 30°C, pH 7.5 Glycine max
4.4.1.5 0.049
-
 glutathione-phenylglyoxal 30°C, pH 7.5 Glycine max
4.4.1.5 0.066
-
 homoglutathione-methylglyoxal 30°C, pH 7.5 Glycine max
4.4.1.5 0.2
-
 gamma-glutamylcysteine-methylglyoxal hemithioacetal 30°C, pH 7.5 Glycine max
4.4.1.5 0.24
-
 gamma-glutamylcysteine-phenylglyoxal hemithioacetal 30°C, pH 7.5 Glycine max

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Zn2+ 4fold higher glyoxalase I activity when glyoxalase is isolated from Zn2+-grown bacteria Glycine max

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 21000
-
 2 * 21000, deduced from nucleotide sequence Glycine max
4.4.1.5 24000
-
 2 * 24000, SDS-PAGE Glycine max
4.4.1.5 38000
-
 gel filtration Glycine max

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.4.1.5 glutathione-methylglyoxal hemithioacetal Glycine max glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione (R)-S-lactoylglutathione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Glycine max Q9ZS21 soybean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5 recombinant glyoxalase I Glycine max

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 gamma-glutamylcysteine-methylglyoxal hemithioacetal gamma-glutamylcysteine-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Glycine max ?
-
 ?
4.4.1.5 gamma-glutamylcysteine-phenylglyoxal hemithioacetal gamma-glutamylcysteine-phenylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Glycine max ?
-
 ?
4.4.1.5 glutathione-methylglyoxal hemithioacetal glutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Glycine max (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione-phenylglyoxal hemithioacetal glutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Glycine max ?
-
 ?
4.4.1.5 homoglutathione-methylglyoxal hemithioacetal homoglutathione-methylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Glycine max (R)-S-lactoylhomoglutathione
-
 ?
4.4.1.5 homoglutathione-phenylglyoxal hemithioacetal homoglutathione-phenylglyoxal hemithioacetal is formed non-enzymatically from methylglyoxal and glutathione Glycine max ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 dimer 2 * 24000, SDS-PAGE Glycine max
4.4.1.5 dimer 2 * 21000, deduced from nucleotide sequence Glycine max

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 glyoxalase I
-
 Glycine max
4.4.1.5 GmGlyox I
-
 Glycine max