EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.71 | captopril | solECE, activity 106.9% | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.24.71 | solECE-1 constructed by fusing the cleavable N-terminalsignal sequence of human alkaline phosphatase in frame with the entire extracellular domain of ECE-1, transfected into CHO-K1 cells | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.24.71 | 1,10-phenanthroline | - |
Homo sapiens | |
3.4.24.71 | EDTA | - |
Homo sapiens | |
3.4.24.71 | additional information | little or no inhibition with captopril | Homo sapiens | |
3.4.24.71 | PD 069185 | - |
Homo sapiens | |
3.4.24.71 | phosphoramidon | - |
Homo sapiens | |
3.4.24.71 | thiorphan | 50% inhibition of native ECE-1, 70% inhibition of solECE-1 | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.71 | 0.0027 | - |
Big endothelin-1 | pH 6.8, 37°C, ECE-1a | Homo sapiens | |
3.4.24.71 | 0.00465 | - |
Big endothelin-1 | pH 6.4, 37°C, solECE-1 | Homo sapiens | |
3.4.24.71 | 0.00751 | - |
Big endothelin-1 | pH 6.4, 37°C, ECE-1a | Homo sapiens | |
3.4.24.71 | 0.0082 | - |
Big endothelin-1 | pH 6.8, 37°C, solECE-1a | Homo sapiens | |
3.4.24.71 | 0.01987 | - |
Big endothelin-1 | pH 7.1, 37°C, ECE-1a | Homo sapiens | |
3.4.24.71 | 0.04831 | - |
Big endothelin-1 | pH 7.1, 37°C, solECE-1a | Homo sapiens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.24.71 | 107600 | - |
2 * 110000, SDS-PAGE, 2 * 107600, MALDI | Homo sapiens |
3.4.24.71 | 110000 | - |
2 * 110000, SDS-PAGE, 2 * 107600, MALDI | Homo sapiens |
3.4.24.71 | 232000 | - |
gel filtration | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.71 | big endothelin-1 + H2O | Homo sapiens | key enzyme in the biosynthesis of the endothelins | endothelin + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.24.71 | Homo sapiens | - |
human | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.24.71 | - |
Homo sapiens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.24.71 | 0.0001799 | - |
recombinant enzyme | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.24.71 | big endothelin-1 + H2O | - |
Homo sapiens | endothelin + ? | - |
? | |
3.4.24.71 | big endothelin-1 + H2O | key enzyme in the biosynthesis of the endothelins | Homo sapiens | endothelin + ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.24.71 | dimer | 2 * 110000, SDS-PAGE, 2 * 107600, MALDI | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.24.71 | ECE-1 | - |
Homo sapiens |
3.4.24.71 | endothelin-converting enzyme-1 | - |
Homo sapiens |
3.4.24.71 | solECE-1 | - |
Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.24.71 | 0.00528 | - |
Big endothelin-1 | pH 6.4, 37°C, ECE-1a | Homo sapiens | |
3.4.24.71 | 0.097 | - |
Big endothelin-1 | pH 6.4, 37°C, solECE-1 | Homo sapiens | |
3.4.24.71 | 0.112 | - |
Big endothelin-1 | pH 6.8, 37°C, ECE-1a | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.71 | 6.1 | 6.4 | solECE-1 | Homo sapiens |
3.4.24.71 | 6.7 | 6.9 | ECE-1 | Homo sapiens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.24.71 | 5.5 | 6.8 | solECE-1 half-maximal activity at pH 5.7 and pH 6.7 | Homo sapiens |
3.4.24.71 | 5.7 | 7.3 | native ECE-1, half-maximal activity at pH 6.2 and pH 7.0 | Homo sapiens |