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Literature summary extracted from

  • Ahn, K.; Herman, S.B.; Fahnoe, D.C.
    Soluble human endothelin-converting enzyme-1: expression, purification, and demonstration of pronounced pH sensitivity (1998), Arch. Biochem. Biophys., 359, 258-268.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.24.71 captopril solECE, activity 106.9% Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.71 solECE-1 constructed by fusing the cleavable N-terminalsignal sequence of human alkaline phosphatase in frame with the entire extracellular domain of ECE-1, transfected into CHO-K1 cells Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.71 1,10-phenanthroline
-
Homo sapiens
3.4.24.71 EDTA
-
Homo sapiens
3.4.24.71 additional information little or no inhibition with captopril Homo sapiens
3.4.24.71 PD 069185
-
Homo sapiens
3.4.24.71 phosphoramidon
-
Homo sapiens
3.4.24.71 thiorphan 50% inhibition of native ECE-1, 70% inhibition of solECE-1 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.71 0.0027
-
Big endothelin-1 pH 6.8, 37°C, ECE-1a Homo sapiens
3.4.24.71 0.00465
-
Big endothelin-1 pH 6.4, 37°C, solECE-1 Homo sapiens
3.4.24.71 0.00751
-
Big endothelin-1 pH 6.4, 37°C, ECE-1a Homo sapiens
3.4.24.71 0.0082
-
Big endothelin-1 pH 6.8, 37°C, solECE-1a Homo sapiens
3.4.24.71 0.01987
-
Big endothelin-1 pH 7.1, 37°C, ECE-1a Homo sapiens
3.4.24.71 0.04831
-
Big endothelin-1 pH 7.1, 37°C, solECE-1a Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.24.71 107600
-
2 * 110000, SDS-PAGE, 2 * 107600, MALDI Homo sapiens
3.4.24.71 110000
-
2 * 110000, SDS-PAGE, 2 * 107600, MALDI Homo sapiens
3.4.24.71 232000
-
gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.71 big endothelin-1 + H2O Homo sapiens key enzyme in the biosynthesis of the endothelins endothelin + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.71 Homo sapiens
-
human
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.71
-
Homo sapiens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.71 0.0001799
-
recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.71 big endothelin-1 + H2O
-
Homo sapiens endothelin + ?
-
?
3.4.24.71 big endothelin-1 + H2O key enzyme in the biosynthesis of the endothelins Homo sapiens endothelin + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.24.71 dimer 2 * 110000, SDS-PAGE, 2 * 107600, MALDI Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.24.71 ECE-1
-
Homo sapiens
3.4.24.71 endothelin-converting enzyme-1
-
Homo sapiens
3.4.24.71 solECE-1
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.24.71 0.00528
-
Big endothelin-1 pH 6.4, 37°C, ECE-1a Homo sapiens
3.4.24.71 0.097
-
Big endothelin-1 pH 6.4, 37°C, solECE-1 Homo sapiens
3.4.24.71 0.112
-
Big endothelin-1 pH 6.8, 37°C, ECE-1a Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.71 6.1 6.4 solECE-1 Homo sapiens
3.4.24.71 6.7 6.9 ECE-1 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.24.71 5.5 6.8 solECE-1 half-maximal activity at pH 5.7 and pH 6.7 Homo sapiens
3.4.24.71 5.7 7.3 native ECE-1, half-maximal activity at pH 6.2 and pH 7.0 Homo sapiens