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Literature summary extracted from

  • Kosugi, A.; Murashima, K.; Doi, R.H.
    Xylanase and acetyl xylan esterase activities of XynA, a key subunit of the Clostridium cellulovorans cellulosome for xylan degradation (2002), Appl. Environ. Microbiol., 68, 6399-6402.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 gene xynA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) as C-terminally His-tagged and N-terminally S-protein tagged enzyme Clostridium cellulovorans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.72 cellulosome enzyme is a key component of the cellulosome performing xylan and cell wall degradation Clostridium cellulovorans 43263
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 57038
-
 x * 57038, DNA sequence calculation Clostridium cellulovorans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.72 acetylated xylan + H2O Clostridium cellulovorans enzyme is a key component of xylan and cell wall degradation xylan + acetate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Clostridium cellulovorans Q8GH59 gene xynA, enzyme with a double specificity as xylanase and acetylxylan esterase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 recombinant tagged protein from Escherichia coli Clostridium cellulovorans

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.72 acetylxylan + 3 H2O = 3 acetic acid + xylan catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin. Clostridium cellulovorans
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.72 121.5
-
 purified recombinant enzyme, substrate acetylated xylan Clostridium cellulovorans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Clostridium cellulovorans 4-nitrophenol + acetate
-
 ?
3.1.1.72 acetylated xylan + H2O acetylated xylan is the best substrate, half maximal activity with birchwood xylan Clostridium cellulovorans xylan + acetate
-
 ?
3.1.1.72 acetylated xylan + H2O enzyme is a key component of xylan and cell wall degradation Clostridium cellulovorans xylan + acetate
-
 ?
3.1.1.72 cellulose acetate + H2O very low activity Clostridium cellulovorans cellulose + acetate
-
 ?
3.1.1.72 D-galactose pentaacetate + H2O low activity Clostridium cellulovorans D-galactose + acetate
-
 ?
3.1.1.72 N,N'-diacetylchitobiose + H2O
-
 Clostridium cellulovorans chitobiose + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 ? x * 57038, DNA sequence calculation Clostridium cellulovorans

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 acetyl xylan esterase
-
 Clostridium cellulovorans
3.1.1.72 xylanase
-
 Clostridium cellulovorans
3.1.1.72 XynA
-
 Clostridium cellulovorans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 37
-
 assay at Clostridium cellulovorans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.72 6
-
 assay at Clostridium cellulovorans