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Literature summary extracted from

  • Zappa, S.; Rolland, J.L.; Flament, D.; Gueguen, Y.; Boudrant, J.; Dietrich, J.
    Characterization of a highly thermostable alkaline phosphatase from the euryarchaeon Pyrococcus abyssi (2001), Appl. Environ. Microbiol., 67, 4504-4511.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.1 Co2+ 2fold activation at 1 mM Pyrococcus abyssi
3.1.3.1 Mg2+ 2fold activation at 1 mM Pyrococcus abyssi
3.1.3.1 Zn2+ 2fold activation at 1 mM Pyrococcus abyssi

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.1 DNA and amino acid sequence determination and analysis, expression in Escherichia coli HMS174 (DE3) Pyrococcus abyssi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.1 2-mercaptoethanol 68% inhibition at 1 mM, 86% inhibition at 10 mM Pyrococcus abyssi
3.1.3.1 Ca2+ 17% inhibition at 1 mM Pyrococcus abyssi
3.1.3.1 Cu2+ 28% inhibition at 1 mM Pyrococcus abyssi
3.1.3.1 DTT nearly complete inhibition at 2 mM Pyrococcus abyssi
3.1.3.1 EDTA 90% inhibition at 1 mM Pyrococcus abyssi
3.1.3.1 EGTA 82% inhibition at 1 mM Pyrococcus abyssi
3.1.3.1 KCl 90% remaining activity Pyrococcus abyssi
3.1.3.1 molybdate slight inhibition at 10 mM Pyrococcus abyssi
3.1.3.1 additional information no inhibition by molybdate and vanadate Pyrococcus abyssi
3.1.3.1 NaCl 2 M, 60% remaining activity Pyrococcus abyssi
3.1.3.1 Ni2+ 56% inhibition at 1 mM Pyrococcus abyssi
3.1.3.1 phosphate
-
 Pyrococcus abyssi
3.1.3.1 vanadate
-
 Pyrococcus abyssi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.1 additional information highly dependent on divalent cations Pyrococcus abyssi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.1 54000
-
 1 * 54000, SDS-PAGE Pyrococcus abyssi
3.1.3.1 54000
-
 2 * 54000, SDS-PAGE Pyrococcus abyssi

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.1 Pyrococcus abyssi Q9UZV2 euryarchaeon, strain Orsay, highly thermostable enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.1 recombinant from Escherichia coli, 450fold, to 95% purity Pyrococcus abyssi

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.1 a phosphate monoester + H2O = an alcohol + phosphate Wide specificity. Also catalyses transphosphorylations. Some enzymes hydrolyse diphosphate Pyrococcus abyssi
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.1 4-nitrophenyl phosphate + H2O
-
 Pyrococcus abyssi 4-nitrophenol + phosphate
-
 ?
3.1.3.1 additional information the enzyme also dephosphorylizes cohesive and blunt ends of DNA fragments, cohesive ends are slightly preferred Pyrococcus abyssi ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.1 dimer 2 * 54000, SDS-PAGE Pyrococcus abyssi
3.1.3.1 monomer 1 * 54000, SDS-PAGE Pyrococcus abyssi
3.1.3.1 More both monomeric and dimeric enzyme forms exist, no cysteine residues are involved in intramolecular disulfide bonds Pyrococcus abyssi

Synonyms

EC Number Synonyms Comment Organism
3.1.3.1 alkaline phosphatase
-
 Pyrococcus abyssi
3.1.3.1 AP
-
 Pyrococcus abyssi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.1 70
-
-
 Pyrococcus abyssi

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.1 37 80 25% of maximal activity at 37°C, 80% of maximal activity at 60-80°C Pyrococcus abyssi

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.1 90
-
 80% remaining activity after 36 h Pyrococcus abyssi
3.1.3.1 100
-
 half-life 18 h Pyrococcus abyssi
3.1.3.1 105
-
 half-life 5 h Pyrococcus abyssi
3.1.3.1 115
-
 inactivation within 30 min Pyrococcus abyssi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.1 11
-
-
 Pyrococcus abyssi

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.3.1 9 12
-
 Pyrococcus abyssi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.3.1 Pyrococcus abyssi calculation from amino acid sequence
-
 4.9