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Literature summary extracted from

  • Qi, H.; Atkinson, I.; Xiao, S.; Choi, Y.J.; Tobimatsu, T.; Shane, B.
    Folylpoly-gamma-glutamate synthetase: generation of isozymes and the role in one carbon metabolism and antifolate cytotoxicity (1999), Adv. Enzyme Regul., 39, 263-273.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.17 expression in Escherichia coli FPGS deficient strain and in CHO AUXB1 cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.17 cytosol a single gene encodes both, mitochondrial and cytosolic isoforms of the enzyme Homo sapiens 5829
-
6.3.2.17 mitochondrion a single gene encodes both, mitochondrial and cytosolic isoforms of the enzyme Homo sapiens 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.17 additional information Homo sapiens the enzyme catalyzes the MgATP-dependent addition of glutamate residues to the gamma-carboxyl of folates to generate folylpolyglutamates ?
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 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.17 Homo sapiens
-
 human
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.17 ATP + methotrexate-Glu + L-glutamate CHO cells expressing the human enzyme accumulate more metabolized drug than CHO cells expressing equivalent levels of endogenous hamster enzyme. Cells expressing only the mitochondrial isozyme do not metabolize this substrate Homo sapiens ADP + methotrexate-Glu2 + phosphate
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 ?
6.3.2.17 additional information mitochondrial enzyme activity is required for mitochondrial folate accumulation, and cells lacking this isozyme are auxotrophic for glycine. Overexpression of cytosolic enzyme does not complement the lack of mitochondrial activity Homo sapiens ?
-
 ?
6.3.2.17 additional information the enzyme catalyzes the MgATP-dependent addition of glutamate residues to the gamma-carboxyl of folates to generate folylpolyglutamates Homo sapiens ?
-
 ?