BRENDA - Enzyme Database

Expression, purification, crystallization and preliminary crystallographic analysis of Leishmania mexicana phosphoglycerate mutase

Poonperm, B.; Guerra, D.G.; McNae, I.W.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.; Acta Crystallogr. Sect. D 59, 1313-1316 (2003)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Leishmania mexicana
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.4.2.12
crystals of iPGAM complexed with 3-phosphoglycerate and Co2+, hanging-drop vapor diffusion, crystallization drops contain equal volumes of reservoir and enzyme solution, 5 mg/ml iPGAM, 1.5 mM 3-phosphoglycerate, 50 mM NaCl, 0.01 mM CoCl2 in 20 mM TEA buffer, pH 7.4, crystals diffract to 1.9 A
Leishmania mexicana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Leishmania mexicana
-
3-phospho-D-glycerate
-
Leishmania mexicana
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.2.12
Leishmania mexicana
Q86N96
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.4.2.12
recombinant iPGAM
Leishmania mexicana
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
649202
Leishmania mexicana
3-phospho-D-glycerate
-
649202
Leishmania mexicana
r
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate-independent iPGAM
Leishmania mexicana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
expression in Escherichia coli
Leishmania mexicana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.4.2.12
additional information
2,3-bisphosphoglycerate-independent iPGAM
Leishmania mexicana
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.4.2.12
crystals of iPGAM complexed with 3-phosphoglycerate and Co2+, hanging-drop vapor diffusion, crystallization drops contain equal volumes of reservoir and enzyme solution, 5 mg/ml iPGAM, 1.5 mM 3-phosphoglycerate, 50 mM NaCl, 0.01 mM CoCl2 in 20 mM TEA buffer, pH 7.4, crystals diffract to 1.9 A
Leishmania mexicana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.4.2.12
2-phospho-D-glycerate
Leishmania mexicana
-
3-phospho-D-glycerate
-
Leishmania mexicana
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.4.2.12
recombinant iPGAM
Leishmania mexicana
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.2.12
2-phospho-D-glycerate
-
649202
Leishmania mexicana
3-phospho-D-glycerate
-
649202
Leishmania mexicana
r