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Literature summary extracted from

  • Poonperm, B.; Guerra, D.G.; McNae, I.W.; Fothergill-Gilmore, L.A.; Walkinshaw, M.D.
    Expression, purification, crystallization and preliminary crystallographic analysis of Leishmania mexicana phosphoglycerate mutase (2003), Acta Crystallogr. Sect. D, 59, 1313-1316.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.12 expression in Escherichia coli Leishmania mexicana

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.2.12 crystals of iPGAM complexed with 3-phosphoglycerate and Co2+, hanging-drop vapor diffusion, crystallization drops contain equal volumes of reservoir and enzyme solution, 5 mg/ml iPGAM, 1.5 mM 3-phosphoglycerate, 50 mM NaCl, 0.01 mM CoCl2 in 20 mM TEA buffer, pH 7.4, crystals diffract to 1.9 A Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.12 2-phospho-D-glycerate Leishmania mexicana
-
 3-phospho-D-glycerate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.12 Leishmania mexicana Q86N96
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.12 recombinant iPGAM Leishmania mexicana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.12 2-phospho-D-glycerate
-
 Leishmania mexicana 3-phospho-D-glycerate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.2.12 iPGAM
-
 Leishmania mexicana

Cofactor

EC Number Cofactor Comment Organism Structure
5.4.2.12 additional information 2,3-bisphosphoglycerate-independent iPGAM Leishmania mexicana