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Literature summary extracted from

  • Lahiri, S.D.; Zhang, G.; Radstrom, P.; Dunaway-Mariano, D.; Allen, K.N.
    Crystallization and preliminary X-ray diffraction studies of beta-phosphoglucomutase from Lactococcus lactus (2002), Acta Crystallogr. Sect. D, 58, 324-326.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.4.2.6 overexpression of native and selenomethionyl beta-PGM in escherichia coli Lactococcus lactis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.4.2.6 beta-PGM is transferred to 1 mM HEPES, pH 7.5, 10 mM MgCl2 and 0.1 mM dithiothreitol, final beta-PGM concentration at 15 mg/ml, crystals are obtained from either 150 mM ammonium acetate, 100 mM trisodium citrate dihydrate, pH 4.5, 25% polyethylene glycol 4000 or 100 mM ammonium fluoride, 16% polyethylene glycol 3350 by hanging-drop vapor diffusion, crystals of selenium methionyl-substituted beta-PGM diffract to 2.3 A Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.2.6 0.0045
-
 beta-D-glucose 1-phosphate in the presence of beta-D-glucose 1,6-diphosphate and Mg2+ Lactococcus lactis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.2.6 Mg2+
-
 Lactococcus lactis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.2.6 beta-D-Glucose 1-phosphate Lactococcus lactis
-
 beta-D-Glucose 6-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
5.4.2.6 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.4.2.6 recombinant native and selenomethionyl beta-PGM Lactococcus lactis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.4.2.6 18
-
 native beta-PGM Lactococcus lactis
5.4.2.6 40
-
 recombinant beta-PGM Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.2.6 beta-D-Glucose 1-phosphate
-
 Lactococcus lactis beta-D-Glucose 6-phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
5.4.2.6 beta-PGM
-
 Lactococcus lactis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.2.6 17
-
 beta-D-glucose 1-phosphate in the presence of beta-D-glucose 1,6-diphosphate and Mg2+ Lactococcus lactis