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Literature summary extracted from

  • Benini, S.; Degrassi, G.; Krastanova, I.; Lamba, D.; Venturi, V.
    Purification, crystallization and preliminary X-ray analysis of an acetylxylan esterase from Bacillus pumilus (2001), Acta Crystallogr. Sect. D, 57, 1906-1907.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.72 corncob in the medium, induces enzyme production Bacillus pumilus
3.1.1.72 xylan induction of enzyme production Bacillus pumilus

Application

EC Number Application Comment Organism
3.1.1.72 medicine possible application of the enzyme in antibiotic production, e.g. in the deacetylation of cephalosporin C Bacillus pumilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.72 DNA and amino acid sequence determination and analysis, expression in Escherichia coli DH5alpha Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 36012
-
 6 * 36012, amino acid calculation and crystal structure Bacillus pumilus
3.1.1.72 190000
-
 about, gel filtration Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Bacillus pumilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.72 recombinant from Escherichia coli DH5alpha, preparation still contains traces of Escherichia coli inorganic pyrophosphatase Bacillus pumilus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.72 acetylxylan + 3 H2O = 3 acetic acid + xylan catalyses the hydrolysis of acetyl groups from polymeric xylan, acetylated xylose, acetylated glucose, alpha-naphthyl acetate, p-nitrophenyl acetate but not from triacetylglycerol. Does not act on acetylated mannan or pectin, enzyme possesses a catalytic triad of Ser-His-Asp, catalytic mechanism Bacillus pumilus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Bacillus pumilus 4-nitrophenol + acetate
-
 ?
3.1.1.72 acetylated xylan + H2O
-
 Bacillus pumilus xylan + acetate
-
 ?
3.1.1.72 cephalosporin C + H2O
-
 Bacillus pumilus deacetylcephalosporin C + acetate
-
 ?
3.1.1.72 D-glucose pentaacetate + H2O
-
 Bacillus pumilus D-glucose + acetate
-
 ?
3.1.1.72 xylose tetraacetate + H2O
-
 Bacillus pumilus xylose + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.72 hexamer 6 * 36012, amino acid calculation and crystal structure Bacillus pumilus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.72 AXE
-
 Bacillus pumilus