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Literature summary extracted from

  • Sri Kannathasan, V.; Staines, A.G.; Dong, C.J.; Field, R.A.; Preston, A.G.; Maskell, D.J.; Naismith, J.H.
    Overexpression, purification, crystallization and data collection on the Bordetella pertussis wlbD gene product, a putative UDP-GlcNAc 2'-epimerase (2001), Acta Crystallogr. Sect. D, 57, 1310-1312.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.1.3.14 overexpression in Escherichia coli Bordetella pertussis
5.1.3.23 overexpression in Escherichia coli Bordetella pertussis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.1.3.14 vapor diffusion method Bordetella pertussis
5.1.3.23 crystals of the mutant Gln339Arg wlbD enzyme obtained by sitting-drop vapour diffusion, uncomplexed Gln339Arg and UDP-GlcNAc complex. Space group as P2(1)2(1)2(1), with unit-cell parameters a = 78, b = 91, c = 125 A, alpha = 78 A, b = 91 A, c = 90 A. The asymmetric unit contains two monomers and 53% solvent Bordetella pertussis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.1.3.14 39368
-
 x * 39368, calculation from nucleotide sequence Bordetella pertussis
5.1.3.14 40000
-
 x * 40000, SDS-PAGE Bordetella pertussis

Organism

EC Number Organism UniProt Comment Textmining
5.1.3.14 Bordetella pertussis
-
-
-
5.1.3.23 Bordetella pertussis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.1.3.14
-
 Bordetella pertussis
5.1.3.23
-
 Bordetella pertussis

Subunits

EC Number Subunits Comment Organism
5.1.3.14 ? x * 40000, SDS-PAGE Bordetella pertussis
5.1.3.14 ? x * 39368, calculation from nucleotide sequence Bordetella pertussis

Synonyms

EC Number Synonyms Comment Organism
5.1.3.14 UDP-GlcNAc 2'-epimerase
-
 Bordetella pertussis
5.1.3.23 WlbD
-
 Bordetella pertussis