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Literature summary extracted from

  • Yan, Y.; Munshi, S.; Li, Y.; Pryor, K.A.; Marsilio, F.; Leiting, B.
    Crystallization and preliminary X-ray analysis of the Escherichia coli UDP-MurNAc-tripeptide D-alanyl-D-alanine-adding enzyme (MurF) (1999), Acta Crystallogr. Sect. D, 55, 2033-2034.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.10 pharmacology attractive target for development of antibacterial agents Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.2.10 expression in strain BL21(DE3) Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.2.10 hanging-drop vapour diffusion method, purified recombinant enzyme 12.2 mg/ml, in 10 mM Tris-HCl, pH 7.4, 10 mM DTT, plus equal volume of reservoir solution: 0.1 M Bis-Tris propane buffer, pH 9.4, 18% PEG 8000, 0.12 M MgSO4, 10% glycerol, about 20 days, X-ray diffraction structure determination at 2.8 A resolution, and analysis Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.10 cytosol
-
 Escherichia coli 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala Escherichia coli enzyme is part of the bacterial peptidoglycan forming group of enzymes ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
 ?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala Escherichia coli K37 enzyme is part of the bacterial peptidoglycan forming group of enzymes ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.10 Escherichia coli P11880
-
-
6.3.2.10 Escherichia coli K37 P11880
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.2.10 recombinant from strain BL21(DE3) Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
 ?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala enzyme is part of the bacterial peptidoglycan forming group of enzymes Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
 ?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala
-
 Escherichia coli K37 ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
 ?
6.3.2.10 ATP + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys + D-Ala-D-Ala enzyme is part of the bacterial peptidoglycan forming group of enzymes Escherichia coli K37 ADP + phosphate + UDP-N-acetylmuramoyl-L-Ala-D-Glu-L-Lys-D-Ala-D-Ala
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.2.10 D-Ala-D-Ala-adding enzyme
-
 Escherichia coli
6.3.2.10 MurF
-
 Escherichia coli
6.3.2.10 Synthetase, uridine diphosphoacetylmuramoylpentapeptide
-
 Escherichia coli
6.3.2.10 UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine synthetase
-
 Escherichia coli
6.3.2.10 UDP-N-acetylmuramoylalanyl-D-glutamyl-lysine-D-alanyl-D-alanine ligase
-
 Escherichia coli
6.3.2.10 UDPacetylmuramoylpentapeptide synthetase
-
 Escherichia coli
6.3.2.10 Uridine diphosphoacetylmuramoylpentapeptide synthetase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.10 ATP
-
 Escherichia coli