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Literature summary extracted from

  • Mohamed, M.E.S.
    Biochemical and molecular characterization of phenylacetate-coenzyme A ligase, an enzyme catalyzing the first step in aerobic metabolism of phenylacetic acid in Azoarcus evansii (2000), J. Bacteriol., 182, 286-294.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.30
-
 Aromatoleum evansii

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.30 Cu2+ 1 mM, more than 80% inhibition Aromatoleum evansii
6.2.1.30 iodoacetamide
-
 Aromatoleum evansii
6.2.1.30 NaF 2 mM; 2 mM, 84% loss of activity Aromatoleum evansii
6.2.1.30 NEM
-
 Aromatoleum evansii
6.2.1.30 Ni2+ 1 mM, more than 80% inhibition Aromatoleum evansii
6.2.1.30 Zn2+ 1 mM, more than 80% inhibition Aromatoleum evansii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.30 0.014
-
 phenylacetate 37°C Aromatoleum evansii
6.2.1.30 0.045
-
 CoA 37°C Aromatoleum evansii
6.2.1.30 0.06
-
 ATP 37°C Aromatoleum evansii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.30 Mg2+ required Aromatoleum evansii
6.2.1.30 Mn2+ 36% of the activation with Mg2+ Aromatoleum evansii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.30 48750
-
 x * 48750, calculation from nucleotide sequence Aromatoleum evansii
6.2.1.30 50000
-
 1 * 50000, SDS-PAGE Aromatoleum evansii
6.2.1.30 52000
-
 gel filtration Aromatoleum evansii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.30 ATP + phenylacetate + CoA Aromatoleum evansii first enzyme involved in the aerobic catabolism of phenylacetic acid ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.30 Aromatoleum evansii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.30
-
 Aromatoleum evansii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.30 0.000076
-
 maximal specific activity in growing cultures Aromatoleum evansii
6.2.1.30 0.48
-
-
 Aromatoleum evansii

Storage Stability

EC Number Storage Stability Organism
6.2.1.30 -20°C, 10% glycerol, activity is retained for 2 months without remarkable loss of activity Aromatoleum evansii
6.2.1.30 4°C, 10% glycerol, 65% of the activity is recovered after 72 h Aromatoleum evansii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.30 ATP + phenylacetate + CoA
-
 Aromatoleum evansii AMP + diphosphate + phenylacetyl-CoA
-
 ?
6.2.1.30 ATP + phenylacetate + CoA first enzyme involved in the aerobic catabolism of phenylacetic acid Aromatoleum evansii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.30 monomer 1 * 50000, SDS-PAGE Aromatoleum evansii
6.2.1.30 monomer x * 48750, calculation from nucleotide sequence Aromatoleum evansii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.30 40
-
 ATP 37°C Aromatoleum evansii
6.2.1.30 40
-
 CoA 37°C Aromatoleum evansii
6.2.1.30 40
-
 phenylacetate 37°C Aromatoleum evansii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.30 8 8.5
-
 Aromatoleum evansii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.30 7 9 pH 9: 55% of maximal activity, pH 9.0: 50% of maximal activity Aromatoleum evansii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.30 Aromatoleum evansii isoelectric focusing
-
 6.1