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Literature summary extracted from
- A phospholipase C from Trypanosoma brucei which selectively cleaves the glycolipid on the variant surface glycoprotein (1986), J. Biol. Chem., 261, 13813-13819.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.14 | DTT | activation, 0.025 M | Trypanosoma brucei |  |
| 4.6.1.14 | EDTA | activation, 5 mM | Trypanosoma brucei | |
| 4.6.1.14 | EGTA | activation, 5 mM | Trypanosoma brucei | |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.14 | Ca2+ | weak, 5 mM | Trypanosoma brucei | |
| 4.6.1.14 | KCl | 0.125 M | Trypanosoma brucei | |
| 4.6.1.14 | Mg2+ | weak, 5 mM | Trypanosoma brucei | |
| 4.6.1.14 | additional information | no inhibition by IAA | Trypanosoma brucei | |
| 4.6.1.14 | NaCl | 0.125 M | Trypanosoma brucei | |
| 4.6.1.14 | NEM | 5 mM | Trypanosoma brucei | |
| 4.6.1.14 | NH4Cl | 0.125 M | Trypanosoma brucei | |
| 4.6.1.14 | p-chloromercuriphenylsulfonic acid | strong, 5 mM | Trypanosoma brucei | |
| 4.6.1.14 | sulfhydryl reagents | - |
Trypanosoma brucei | |
| 4.6.1.14 | Zn2+ | strong, 5 mM | Trypanosoma brucei | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.6.1.14 | membrane | - |
Trypanosoma brucei | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.6.1.14 | Ca2+ | not | Trypanosoma brucei | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.14 | 37000 | - |
1 * 37000, SDS-PAGE | Trypanosoma brucei |
| 4.6.1.14 | 47000 | - |
gel filtration, sedimentation equilibrium centrifugation, in the presence of 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonic acid | Trypanosoma brucei |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.6.1.14 | Trypanosoma brucei | - |
ILTat 1.3, isolated from blood of Swiss mice or Wistar rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.6.1.14 | solubilized by n-octyl glucoside | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.14 | 11000000 | - |
concentrated CM-Sephadex pool | Trypanosoma brucei |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.6.1.14 | -70°C, in crude membranes, 6 months | Trypanosoma brucei |
| 4.6.1.14 | -70°C, purified enzyme preparation, at least 2 months | Trypanosoma brucei |
| 4.6.1.14 | 4°C, 30% loss within 5 days, 10% loss of activity in the presence of 50% glycerol | Trypanosoma brucei |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.6.1.14 | 1,2-dimyristoyl-sn-phosphatidylinositol + H2O | poor substrate | Trypanosoma brucei | ? | - |
? | |
| 4.6.1.14 | lipid A | biological precursor of variant-surface-glycoprotein glycolipid | Trypanosoma brucei | ? | - |
? | |
| 4.6.1.14 | additional information | no substrate is 1-stearoyl-2-arachidonoyl-sn-phosphatidylinositol | Trypanosoma brucei | ? | - |
? | |
| 4.6.1.14 | variant-surface-glycoprotein | highly specific | Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | i.e. 1,2-dimyristoylglycerol | ? | |
| 4.6.1.14 | variant-surface-glycoprotein | substrate is tethered to cell membrane by glycolipid moiety containing 1,2-dimyristoyl-sn-phosphatidylinositol | Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | i.e. 1,2-dimyristoylglycerol | ? | |
| 4.6.1.14 | variant-surface-glycoprotein | phospholipase C-type hydrolysis | Trypanosoma brucei | 1,2-didecanoylglycerol + soluble variant-surface-glycoprotein | i.e. 1,2-dimyristoylglycerol | ? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.6.1.14 | monomer | 1 * 37000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.14 | 37 | - |
assay at | Trypanosoma brucei |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.14 | 37 | - |
10 min, stable | Trypanosoma brucei |
| 4.6.1.14 | 50 | - |
10 min, inactivation | Trypanosoma brucei |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.14 | 7.5 | 8.5 | - |
Trypanosoma brucei |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.6.1.14 | 6.5 | 9.5 | about half-maximal activity at pH 6.5 and 9.5, little or no activity below pH 6 | Trypanosoma brucei |
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