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Literature summary extracted from

  • Stourman, N.V.; Rose, J.H.; Vuilleumier, S.; Armstrong, R.N.
    Catalytic mechanism of dichloromethane dehalogenase from Methylophilus sp. strain DM11 (2003), Biochemistry, 42, 11048-11056.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.5.1.3 glutathione
-
 Methylophilus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.5.1.3
-
 Methylophilus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.3 0.00162
-
 CH2BrCl 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.0017
-
 Diiodomethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.002
-
 CH2ClI 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.0031
-
 Dibromomethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.031
-
 Dichloromethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.039
-
 Iodoethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.053
-
 CH2ClF 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.33
-
 chloroethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.33
-
 Bromoethane 25°C, pH 8 Methylophilus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.3 dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Methylophilus sp. P43387
-
-
4.5.1.3 Methylophilus sp. DM11 P43387
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.5.1.3 strain DM11 Methylophilus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 bromoethane + H2O
-
 Methylophilus sp. ethanal + Br-
-
 ?
4.5.1.3 bromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
4.5.1.3 CH2BrCl + H2O
-
 Methylophilus sp. formaldehyde + Br- + Cl-
-
 ?
4.5.1.3 CH2BrCl + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br- + Cl-
-
 ?
4.5.1.3 CH2ClF + H2O
-
 Methylophilus sp. formaldehyde + Cl- + F-
-
 ?
4.5.1.3 CH2ClF + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl- + F-
-
 ?
4.5.1.3 CH2ClI + H2O
-
 Methylophilus sp. formaldehyde + Cl- + I-
-
 ?
4.5.1.3 chloroethane + H2O
-
 Methylophilus sp. ethanal + Cl-
-
 ?
4.5.1.3 dibromomethane + H2O
-
 Methylophilus sp. formaldehyde + Br-
-
 ?
4.5.1.3 dibromomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Br-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?
4.5.1.3 diiodomethane + H2O
-
 Methylophilus sp. formaldehyde + I-
-
 ?
4.5.1.3 diiodomethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + I-
-
 ?
4.5.1.3 iodoethane + H2O
-
 Methylophilus sp. ethanal + I-
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5.1.3 0.009
-
 CH2ClF 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.1
-
 chloroethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.14
-
 Bromomethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 0.15
-
 Iodoethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 1
-
 CH2BrCl 25°C, pH 8 Methylophilus sp.
4.5.1.3 1.03
-
 CH2ClI 25°C, pH 8 Methylophilus sp.
4.5.1.3 1.31
-
 Diiodomethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 1.51
-
 Dichloromethane 25°C, pH 8 Methylophilus sp.
4.5.1.3 3.18
-
 Dibromomethane 25°C, pH 8 Methylophilus sp.