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Literature summary extracted from

  • Gisi, D.; Willi, L.; Traber, H.; Leisinger, T.; Vuilleumier, S.
    Effects of bacterial host and dichloromethane dehalogenase on the competitiveness of methylotrophic bacteria growing with dichloromethane (1998), Appl. Environ. Microbiol., 64, 1194-1202.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.5.1.3
-
 Methylophilus sp.
4.5.1.3 expression in Escherichia coli Methylorubrum extorquens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.5.1.3 0.009
-
 Dichloromethane
-
 Methylorubrum extorquens
4.5.1.3 0.059
-
 Dichloromethane
-
 Methylophilus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.5.1.3 dichloromethane + H2O Methylorubrum extorquens
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylophilus sp.
-
 formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O Methylophilus sp. DM11
-
 formaldehyde + Cl-
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.5.1.3 Methylophilus sp. P43387
-
-
4.5.1.3 Methylophilus sp. DM11 P43387
-
-
4.5.1.3 Methylorubrum extorquens P21161
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.5.1.3 dichloromethane + H2O
-
 Methylorubrum extorquens formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. formaldehyde + Cl-
-
 ?
4.5.1.3 dichloromethane + H2O
-
 Methylophilus sp. DM11 formaldehyde + Cl-
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.5.1.3 0.6
-
 Dichloromethane
-
 Methylorubrum extorquens
4.5.1.3 3.3
-
 Dichloromethane
-
 Methylophilus sp.