Any feedback?
Literature summary extracted from
- Selenocysteine beta-lyase (porcine) (1987), Methods Enzymol., 143, 415-418.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.4.1.16 | 1% bovine serum albumin, 20% sucrose or 20% glycerol protects against inactivation, highly diluted samples sensitive to freezing | Sus scrofa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.16 | L-cysteine | - |
Sus scrofa |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.16 | 0.83 | - |
L-selenocysteine | pH 8.5, 37°C | Sus scrofa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.16 | 48000 | - |
SDS-PAGE | Sus scrofa |
| 4.4.1.16 | 85000 | - |
sedimentation equilibrium centrifugation | Sus scrofa |
| 4.4.1.16 | 93000 | - |
gel filtration | Sus scrofa |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.16 | L-selenocysteine + reduced acceptor | Sus scrofa | - |
selenide + L-alanine + acceptor | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.16 | Sus scrofa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.4.1.16 | - |
Sus scrofa |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.4.1.16 | liver | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.16 | 37 | - |
purified enzyme, pH 8.5, 37°C | Sus scrofa |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.4.1.16 | -20°C, 20% sucrose, stable | Sus scrofa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.16 | L-selenocysteine + reduced acceptor | - |
Sus scrofa | selenide + L-alanine + acceptor | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.16 | dimer | 2 * 48000, SDS-PAGE | Sus scrofa |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.16 | pyridoxal 5'-phosphate | required | Sus scrofa | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.16 | 1 | - |
L-cysteine | pH 8.5, 37°C | Sus scrofa | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
