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Literature summary extracted from

  • de Waal, A.; Meijer, A.H.; Verpoorte, R.
    Strictosidine synthase from Catharanthus roseus: purification and characterization of multiple forms (1995), Biochem. J., 306, 571-580.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.3.3.2 bovine serum albumin 1 mg/ml stimulates activity Catharanthus roseus

General Stability

EC Number General Stability Organism
4.3.3.2 unstable when highly diluted, 1 M sucrose or 1 mg/ml bovine serum albumin stabilizes activity Catharanthus roseus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.2 additional information no substrate inhibition Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.2 0.009
-
 tryptamine pH 6.8, 30°C Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.3.2 tryptamine + secologanin Catharanthus roseus
-
 3-alpha(S)-strictosidine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Catharanthus roseus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.3.3.2 glycoprotein all isoforms, mannose bound to enzyme Catharanthus roseus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2 six isoforms purified Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.3.2 cell culture
-
 Catharanthus roseus
-
4.3.3.2 leaf
-
 Catharanthus roseus
-
4.3.3.2 seed
-
 Catharanthus roseus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3.3.2 31.62
-
 purified enzyme, pH 6.8, 30°C Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 tryptamine + secologanin
-
 Catharanthus roseus 3-alpha(S)-strictosidine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.2 6
-
-
 Catharanthus roseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.3.3.2 5 7.5 50% activity Catharanthus roseus