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Literature summary extracted from

  • Takeuchi, M.; Neyazaki, K.; Matsui, K.
    Chemical modification by 2,4,6-trinitrobenzenesulfonic acid (TNBS) of an essential amino group in 3-ketovalidoxylamine A C-N lyase (1990), Chem. Pharm. Bull., 38, 1419-1420.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.1 2,4,6-Trinitrobenzenesulfonic acid modification of amino group, 4-nitrophenyl-3-ketovalidamine protect, no protection by 4-nitrophenylvalidamine Flavobacterium saccharophilum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.1 0.24
-
 N-4-nitrophenyl-3-ketovalidamine pH 8, 37°C Flavobacterium saccharophilum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.3.1 36000
-
-
 Flavobacterium saccharophilum

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.1 Flavobacterium saccharophilum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.1 N-4-nitrophenyl-3-ketovalidamine
-
 Flavobacterium saccharophilum 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one
-
 ?
4.3.3.1 O-4-nitrophenyl-alpha-D-3-ketoglucoside best substrate, C-O-lyase reaction Flavobacterium saccharophilum 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3.3.1 9
-
-
 Flavobacterium saccharophilum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3.3.1 0.006
-
 2,4,6-Trinitrobenzenesulfonic acid pH 9.5, 30°C, 10% glycerol, second order kinetics Flavobacterium saccharophilum