Any feedback?
Literature summary extracted from
- Purification and properties of 3-ketovalidoxylamine A C-N lyase from Flavobacterium saccharophilum (1985), J. Biochem., 98, 1631-1638.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.3.3.1 | glycerol stabilizes | Flavobacterium saccharophilum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.3.1 | Co2+ | at 1 mM, 37.4% inhibition | Flavobacterium saccharophilum |  |
| 4.3.3.1 | Cu2+ | at 1 mM, 13.5% inhibition | Flavobacterium saccharophilum | |
| 4.3.3.1 | EDTA | i.e. ethylenediamine tetra acetic acid, at 1 mM, 37°C, for 20 min., 100% inhibition, Ca2+ reverses, not Mg2+ | Flavobacterium saccharophilum | |
| 4.3.3.1 | EGTA | Ca2+ reverses; i.e. ethyleneglycol bis(beta-aminoethylether)-N,N'-tetraacetic acid, at 1 mM, 37°C, for 20 min., 100% inhibition | Flavobacterium saccharophilum | |
| 4.3.3.1 | La3+ | at 1 mM, 97.4% inhibition | Flavobacterium saccharophilum | |
| 4.3.3.1 | Mn2+ | at 1 mM, 92.8% inhibition | Flavobacterium saccharophilum | |
| 4.3.3.1 | additional information | at 1 mM, 37°C, for 20 min., no inhibited by p-chloromercuribenzoate and Mg2+, insignificant inhibition by Hg2+ | Flavobacterium saccharophilum | |
| 4.3.3.1 | Sr2+ | at 1 mM, 69.0% inhibition | Flavobacterium saccharophilum | |
| 4.3.3.1 | Zn2+ | at 1 mM, 55.5% inhibition | Flavobacterium saccharophilum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.3.3.1 | 0.24 | - |
N-4-nitrophenyl-3-ketovalidamine | pH 8, 37°C | Flavobacterium saccharophilum | |
| 4.3.3.1 | 0.5 | - |
p-nitrophenyl-alpha-D-3-ketoglucoside | pH 7, 37°C | Flavobacterium saccharophilum | |
| 4.3.3.1 | 2.5 | - |
N-4-Nitrophenyl-3-ketovalienamine | pH 7, 37°C | Flavobacterium saccharophilum | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.3.3.1 | membrane | - |
Flavobacterium saccharophilum | 16020 | - |
| 4.3.3.1 | soluble | - |
Flavobacterium saccharophilum | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.3.3.1 | Ca2+ | requirement | Flavobacterium saccharophilum | |
| 4.3.3.1 | Ca2+ | positive cooperative interaction, maximal activation at 0.003 mM, kinetics | Flavobacterium saccharophilum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.1 | 36000 | - |
gel filtration | Flavobacterium saccharophilum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.3.3.1 | Flavobacterium saccharophilum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.3.3.1 | from soluble fraction, by ammonium sulfate fractionation, column chromatography on CM cellulose and gel filtration on Sephacryl S-200 | Flavobacterium saccharophilum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.1 | 10.73 | - |
- |
Flavobacterium saccharophilum |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.3.3.1 | 4°C, in 0.05 M phosphate buffer, pH 7, 60% loss of activity within 2 weeks, 2 weeks stable in the presence of 10% glycerol | Flavobacterium saccharophilum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.3.3.1 | additional information | the enzyme has C-O-lyase activity | Flavobacterium saccharophilum | ? | - |
? | |
| 4.3.3.1 | additional information | no substrates are O-4-nitrophenyl-beta-D-3-ketoglucoside, N-4-nitrophenylvalidamine, N-4-nitrophenyl-1-epi-3-ketovalidamine, O-4-nitrophenyl-alpha-D-glucoside | Flavobacterium saccharophilum | ? | - |
? | |
| 4.3.3.1 | additional information | no substrates are N-4-nitrophenylvalienamine, O-4-nitrophenyl-beta-D-glucoside | Flavobacterium saccharophilum | ? | - |
? | |
| 4.3.3.1 | N-4-nitrophenyl-3-ketovalidamine | synthetic model compound | Flavobacterium saccharophilum | 4-nitroaniline + 5-D-(5/6)-5-C-(hydroxymethyl)-2,6-dihydroxy-2-cyclohexen-1-one | - |
? | |
| 4.3.3.1 | N-4-nitrophenyl-3-ketovalienamine | synthetic model compound | Flavobacterium saccharophilum | 4-nitroaniline + ? | - |
? | |
| 4.3.3.1 | O-4-nitrophenyl-alpha-D-3-ketoglucoside | synthetic model compound | Flavobacterium saccharophilum | 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose | - |
? | |
| 4.3.3.1 | O-4-nitrophenyl-alpha-D-3-ketoglucoside | best substrate, C-O-lyase reaction | Flavobacterium saccharophilum | 4-nitrophenol + 1,5-anhydro-D-erythro-hex-1-en-3-ulose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.3.3.1 | monomer | 1 * 36000, SDS-PAGE | Flavobacterium saccharophilum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.1 | 40 | - |
- |
Flavobacterium saccharophilum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.1 | 9 | - |
- |
Flavobacterium saccharophilum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.3.3.1 | 5 | 11 | no activity below pH 5 and above pH 11 | Flavobacterium saccharophilum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
