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Literature summary extracted from

  • Chen, X.Y.; Wang, M.; Chen, Y.; Davisson, V.J.; Heinstein, P.
    Cloning and heterologous expression of a second (+)-delta-cadinene synthase from Gossypium arboreum (1996), J. Nat. Prod., 59, 944-951.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.13 expression in Escherichia coli of the CAD1-A isoenzyme, 80% of homology with CAD1-C isoenzyme Gossypium arboreum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.13 0.007
-
 2-trans,6-trans-farnesyl diphosphate isoenzyme CAD1-A, pH 8.7, 30°C Gossypium arboreum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.13 Mg2+ 2.5 mM MgCl2 required for optimal activity, enzyme form CAD1-A Gossypium arboreum
4.2.3.13 Mg2+ 15 mM MgCl2 required for optimal activity, enzyme form CAD1-C Gossypium arboreum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.13 64060
-
 calculated from DNA sequence, CAD1-A isozyme Gossypium arboreum

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.13 Gossypium arboreum
-
 cv. Nanking
-
4.2.3.13 Gossypium arboreum
-
 two enzyme forms CAD1-A and CAD1-C1
-
4.2.3.13 Gossypium arboreum
-
 tree cotton
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.13 CAD1-A isoenzyme Gossypium arboreum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.13 0.021
-
 CAD1-A isozyme, expressed in Escherichia coli Gossypium arboreum
4.2.3.13 0.036
-
 CAD1-A isozyme, removal of 15 amino acids by thrombin cleavage Gossypium arboreum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.13 2-trans,6-trans-farnesyl diphosphate
-
 Gossypium arboreum (+)-delta-cadinene + diphosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.13 7 7.5 enzyme form CAD1-C Gossypium arboreum
4.2.3.13 8.7
-
 enzyme form CAD1-A Gossypium arboreum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.13 6.8 8.7 62% of maximal activity at pH 6.8, enzyme form CAD1-A Gossypium arboreum
4.2.3.13 7 8.7 60% of maximal activity at pH 8.7, enzyme form CAD1-C Gossypium arboreum