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Literature summary extracted from

  • Zhao, G.; Xia, T.; Fischer, R.S.; Jensen, R.A.
    Cyclohexadienyl dehydratase from Pseudomonas aeruginosa. Molecular cloning of the gene and characterization of the gene product (1992), J. Biol. Chem., 267, 2487-2493.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.91
-
 Pseudomonas aeruginosa
4.2.1.91 expression in Escherichia coli Pseudomonas aeruginosa
4.2.1.91 pheC cloned and expressed by functional complementation of a pheA auxotroph of Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.91 L-arogenate competitive inhibition of prephenate dehydratase activity; competitive to prephenate; reaction with prephenate is inhibited Pseudomonas aeruginosa
4.2.1.91 prephenate competitive inhibition; competitive to L-arogenate; reaction with L-arogenate is inhibited Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.91 0.19
-
 L-arogenate enzyme purified directly from Pseudomonas aeruginosa Pseudomonas aeruginosa
4.2.1.91 0.22
-
 L-arogenate pH 7.5, 37°C Pseudomonas aeruginosa
4.2.1.91 0.22
-
 L-arogenate pH 7.5, 22°C Pseudomonas aeruginosa
4.2.1.91 0.22
-
 L-arogenate recombinant protein, purified from Escherichia coli Pseudomonas aeruginosa
4.2.1.91 0.4
-
 prephenate enzyme purified directly from Pseudomonas aeruginosa Pseudomonas aeruginosa
4.2.1.91 0.42
-
 prephenate pH 7.5, 37°C Pseudomonas aeruginosa
4.2.1.91 0.42
-
 prephenate pH 7.5, 22°C Pseudomonas aeruginosa
4.2.1.91 0.42
-
 prephenate recombinant protein, purified from Escherichia coli Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.91 29500
-
 2 * 29500, SDS-PAGE, homodimer Pseudomonas aeruginosa
4.2.1.91 29500
-
 2 * 29500, SDS-PAGE, gel filtration Pseudomonas aeruginosa
4.2.1.91 29500
-
 2 * 30480, calculated, 2 * 29500, SDS-PAGE Pseudomonas aeruginosa
4.2.1.91 30480
-
 calculated from cloned gene pheC Pseudomonas aeruginosa
4.2.1.91 30480
-
 2 * 30480, calculated from amino acis sequence Pseudomonas aeruginosa
4.2.1.91 72000
-
 gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.91 L-arogenate Pseudomonas aeruginosa biosynthetic pathway of phenylalanine L-phenylalanine + CO2 + H2O
-
 ?
4.2.1.91 L-arogenate Pseudomonas aeruginosa
-
 L-phenylalanine + H2O + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.91 Pseudomonas aeruginosa
-
-
-
4.2.1.91 Pseudomonas aeruginosa Q01269
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.91 native and recombinant enzyme Pseudomonas aeruginosa
4.2.1.91 recombinant protein Pseudomonas aeruginosa
4.2.1.91 to electrophoretic homogeneity Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.91 0.0483
-
 L-arogenate dehydratase activity Pseudomonas aeruginosa
4.2.1.91 0.158
-
 prephenate dehydratase activity Pseudomonas aeruginosa
4.2.1.91 102.8
-
 purified recombinant enzyme, with L-arogenate as substrate Pseudomonas aeruginosa
4.2.1.91 307.7
-
 purified recombinant enzyme, with prephenate as substrate Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.91 L-arogenate biosynthetic pathway of phenylalanine Pseudomonas aeruginosa L-phenylalanine + CO2 + H2O
-
 ?
4.2.1.91 L-arogenate
-
 Pseudomonas aeruginosa L-phenylalanine + H2O + CO2
-
 ?
4.2.1.91 additional information specific activity ratio of prephenate dehydratase to arogenate dehydratase activity is 3:1 Pseudomonas aeruginosa ?
-
 ?
4.2.1.91 prephenate pH 7.5, 37°C Pseudomonas aeruginosa phenylpyruvate + H2O + CO2
-
 ?
4.2.1.91 prephenate
-
 Pseudomonas aeruginosa phenylpyruvate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.91 dimer 2 * 29500, SDS-PAGE, homodimer Pseudomonas aeruginosa
4.2.1.91 dimer 2 * 29500, SDS-PAGE, gel filtration Pseudomonas aeruginosa
4.2.1.91 dimer 2 * 30480, calculated from amino acis sequence Pseudomonas aeruginosa
4.2.1.91 dimer 2 * 30480, calculated, 2 * 29500, SDS-PAGE Pseudomonas aeruginosa

Synonyms

EC Number Synonyms Comment Organism
4.2.1.91 arogenate dehydratase
-
 Pseudomonas aeruginosa
4.2.1.91 cyclohexadienyl dehydratase
-
 Pseudomonas aeruginosa
4.2.1.91 prephenate dehydratase
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.91 0.2
-
 L-arogenate
-
 Pseudomonas aeruginosa
4.2.1.91 0.2
-
 L-arogenate pH 7.5, 37°C Pseudomonas aeruginosa
4.2.1.91 0.2
-
 L-arogenate pH 7.5, 22°C Pseudomonas aeruginosa
4.2.1.91 0.4
-
 prephenate
-
 Pseudomonas aeruginosa
4.2.1.91 0.4
-
 prephenate pH 7.5, 37°C Pseudomonas aeruginosa
4.2.1.91 0.4
-
 prephenate pH 7.5, 22°C Pseudomonas aeruginosa