Data extracted from this reference:
General Stability
4.2.1.84
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Inhibitors
4.2.1.84
Ag+
0.01-1 mM AgNO3
Rhodococcus rhodochrous
4.2.1.84
diethyldithiocarbamate
0.1 mM
Rhodococcus rhodochrous
4.2.1.84
Hg2+
HgCl2, 0.01-1 mM
Rhodococcus rhodochrous
4.2.1.84
hydroxylamine
1 mM
Rhodococcus rhodochrous
4.2.1.84
KCN
0.01 mM
Rhodococcus rhodochrous
4.2.1.84
Na2S2O4
1 mM
Rhodococcus rhodochrous
4.2.1.84
phenylhydrazine
1 mM
Rhodococcus rhodochrous
4.2.1.84
Semicarbazide
1 mM, weak inhibition
Rhodococcus rhodochrous
KM Value [mM]
4.2.1.84
1.85
Isobutyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
1.89
acrylonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
1.92
Propionitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
4.88
Crotononitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
6.76
Methacrylonitrile
pH 7.0, 20ºC
Rhodococcus rhodochrous
4.2.1.84
10
Cyanopyrazine
pH 7.0, 20ºC
Rhodococcus rhodochrous
4.2.1.84
12.2
Chloroacetonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
18.5
4-cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
21.7
2-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
21.7
n-butyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
28.6
Benzonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
200
3-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
Metals/Ions
4.2.1.84
Co2+
contains approximately 11-12 mol cobalt/mol enzyme
Rhodococcus rhodochrous
Molecular Weight [Da]
4.2.1.84
26000
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
4.2.1.84
29000
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
4.2.1.84
505000
530000
sedimentation equilibrium method, gel permeation HPLC
Rhodococcus rhodochrous
Organism
4.2.1.84
Rhodococcus rhodochrous
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase
4.2.1.84
Rhodococcus rhodochrous J1
2 kinds of enzyme, a high molecular mass: H-NHase, and a low molecular mass enzyme: L-NHase
Purification (Commentary)
4.2.1.84
; ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg]
4.2.1.84
additional information
Rhodococcus rhodochrous
4.2.1.84
371
purified enzyme
Rhodococcus rhodochrous
Storage Stability
4.2.1.84
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Substrates and Products (Substrate)
4.2.1.84
2-cyanopyridine + H2O
648439
Rhodococcus rhodochrous
pyridine-2-carbamide
?
4.2.1.84
3-cyanopyridine + H2O
648439
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648439
Rhodococcus rhodochrous
?
4.2.1.84
4-cyanopyridine + H2O
648439
Rhodococcus rhodochrous
pyridine-4-carbamide
?
4.2.1.84
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous
acetamide
?
4.2.1.84
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous J1
acetamide
?
4.2.1.84
acrylonitrile + H2O
648439
Rhodococcus rhodochrous
2-propenoic acid amide
i.e. acrylic acid amide
648439
Rhodococcus rhodochrous
?
4.2.1.84
benzonitrile + H2O
648439
Rhodococcus rhodochrous
benzoic acid amide
?
4.2.1.84
chloroacetonitrile + H2O
648439
Rhodococcus rhodochrous
chloroacetamide
?
4.2.1.84
chloroacetonitrile + H2O
648439
Rhodococcus rhodochrous J1
chloroacetamide
?
4.2.1.84
crotononitrile + H2O
19% of the activity with propionitrile
648439
Rhodococcus rhodochrous
(E)-2-butenoic acid amide
?
4.2.1.84
cyanopyrazine + H2O
648439
Rhodococcus rhodochrous
pyrazincarbamide
?
4.2.1.84
cyclopropylcyanide + H2O
648439
Rhodococcus rhodochrous
?
?
4.2.1.84
ethylene cyanhydrine + H2O
648439
Rhodococcus rhodochrous
?
?
4.2.1.84
methacrylonitrile + H2O
648439
Rhodococcus rhodochrous
methylacrylic acid amide
?
4.2.1.84
additional information
specificity overview
648439
Rhodococcus rhodochrous
?
4.2.1.84
additional information
specificity overview
648439
Rhodococcus rhodochrous J1
?
4.2.1.84
n-butyronitrile + H2O
648439
Rhodococcus rhodochrous
n-butyric acid amide
?
4.2.1.84
n-butyronitrile + H2O
648439
Rhodococcus rhodochrous J1
n-butyric acid amide
?
4.2.1.84
propionitrile + H2O
648439
Rhodococcus rhodochrous
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648439
Rhodococcus rhodochrous J1
propionic acid amide
?
Subunits
4.2.1.84
?
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
Temperature Optimum [°C]
4.2.1.84
35
Rhodococcus rhodochrous
Temperature Stability [°C]
4.2.1.84
50
stable up to, 30 min, pH 7.0, 44 mM n-butyric acid
Rhodococcus rhodochrous
pH Optimum
4.2.1.84
6.5
6.8
Rhodococcus rhodochrous
pH Range
4.2.1.84
5
8
about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8
Rhodococcus rhodochrous
pH Stability
4.2.1.84
6
9
20°C, 60 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0; 25°C, 10 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0
Rhodococcus rhodochrous
General Stability (protein specific)
4.2.1.84
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Inhibitors (protein specific)
4.2.1.84
Ag+
0.01-1 mM AgNO3
Rhodococcus rhodochrous
4.2.1.84
diethyldithiocarbamate
0.1 mM
Rhodococcus rhodochrous
4.2.1.84
Hg2+
HgCl2, 0.01-1 mM
Rhodococcus rhodochrous
4.2.1.84
hydroxylamine
1 mM
Rhodococcus rhodochrous
4.2.1.84
KCN
0.01 mM
Rhodococcus rhodochrous
4.2.1.84
Na2S2O4
1 mM
Rhodococcus rhodochrous
4.2.1.84
phenylhydrazine
1 mM
Rhodococcus rhodochrous
4.2.1.84
Semicarbazide
1 mM, weak inhibition
Rhodococcus rhodochrous
KM Value [mM] (protein specific)
4.2.1.84
1.85
Isobutyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
1.89
acrylonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
1.92
Propionitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
4.88
Crotononitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
6.76
Methacrylonitrile
pH 7.0, 20ºC
Rhodococcus rhodochrous
4.2.1.84
10
Cyanopyrazine
pH 7.0, 20ºC
Rhodococcus rhodochrous
4.2.1.84
12.2
Chloroacetonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
18.5
4-cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
21.7
2-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
21.7
n-butyronitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
28.6
Benzonitrile
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
4.2.1.84
200
3-Cyanopyridine
pH 7.0, 20ºC, H-NHase
Rhodococcus rhodochrous
Metals/Ions (protein specific)
4.2.1.84
Co2+
contains approximately 11-12 mol cobalt/mol enzyme
Rhodococcus rhodochrous
Molecular Weight [Da] (protein specific)
4.2.1.84
26000
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
4.2.1.84
29000
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
4.2.1.84
505000
530000
sedimentation equilibrium method, gel permeation HPLC
Rhodococcus rhodochrous
Purification (Commentary) (protein specific)
4.2.1.84
; ammonium sulfate fractionation, chromatography on DEAE-Sephacel, phenyl-Sepharose and Sephacryl S-300, 3.31fold purification
Rhodococcus rhodochrous
Specific Activity [micromol/min/mg] (protein specific)
4.2.1.84
additional information
Rhodococcus rhodochrous
4.2.1.84
371
purified enzyme
Rhodococcus rhodochrous
Storage Stability (protein specific)
4.2.1.84
organic acids stabilize, stable for more than 1 month in 0.1 M HEPES/KOH, pH 7.2, with 44 mM n-butyric acid, n-valeric acid, isovaleric acid, isobutyric acid or n-caproic acid
Rhodococcus rhodochrous
Substrates and Products (Substrate) (protein specific)
4.2.1.84
2-cyanopyridine + H2O
648439
Rhodococcus rhodochrous
pyridine-2-carbamide
?
4.2.1.84
3-cyanopyridine + H2O
648439
Rhodococcus rhodochrous
pyridine-3-carbamide
i.e. nicotinamide
648439
Rhodococcus rhodochrous
?
4.2.1.84
4-cyanopyridine + H2O
648439
Rhodococcus rhodochrous
pyridine-4-carbamide
?
4.2.1.84
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous
acetamide
?
4.2.1.84
acetonitrile + H2O
best substrate
648439
Rhodococcus rhodochrous J1
acetamide
?
4.2.1.84
acrylonitrile + H2O
648439
Rhodococcus rhodochrous
2-propenoic acid amide
i.e. acrylic acid amide
648439
Rhodococcus rhodochrous
?
4.2.1.84
benzonitrile + H2O
648439
Rhodococcus rhodochrous
benzoic acid amide
?
4.2.1.84
chloroacetonitrile + H2O
648439
Rhodococcus rhodochrous
chloroacetamide
?
4.2.1.84
chloroacetonitrile + H2O
648439
Rhodococcus rhodochrous J1
chloroacetamide
?
4.2.1.84
crotononitrile + H2O
19% of the activity with propionitrile
648439
Rhodococcus rhodochrous
(E)-2-butenoic acid amide
?
4.2.1.84
cyanopyrazine + H2O
648439
Rhodococcus rhodochrous
pyrazincarbamide
?
4.2.1.84
cyclopropylcyanide + H2O
648439
Rhodococcus rhodochrous
?
?
4.2.1.84
ethylene cyanhydrine + H2O
648439
Rhodococcus rhodochrous
?
?
4.2.1.84
methacrylonitrile + H2O
648439
Rhodococcus rhodochrous
methylacrylic acid amide
?
4.2.1.84
additional information
specificity overview
648439
Rhodococcus rhodochrous
?
4.2.1.84
additional information
specificity overview
648439
Rhodococcus rhodochrous J1
?
4.2.1.84
n-butyronitrile + H2O
648439
Rhodococcus rhodochrous
n-butyric acid amide
?
4.2.1.84
n-butyronitrile + H2O
648439
Rhodococcus rhodochrous J1
n-butyric acid amide
?
4.2.1.84
propionitrile + H2O
648439
Rhodococcus rhodochrous
propionic acid amide
?
4.2.1.84
propionitrile + H2O
648439
Rhodococcus rhodochrous J1
propionic acid amide
?
Subunits (protein specific)
4.2.1.84
?
x * 26000, alpha, + x * 29000, beta, SDS-PAGE
Rhodococcus rhodochrous
Temperature Optimum [°C] (protein specific)
4.2.1.84
35
Rhodococcus rhodochrous
Temperature Stability [°C] (protein specific)
4.2.1.84
50
stable up to, 30 min, pH 7.0, 44 mM n-butyric acid
Rhodococcus rhodochrous
pH Optimum (protein specific)
4.2.1.84
6.5
6.8
Rhodococcus rhodochrous
pH Range (protein specific)
4.2.1.84
5
8
about 50% of activity maximum at pH 5, about 60% of activity maximum at pH 8
Rhodococcus rhodochrous
pH Stability (protein specific)
4.2.1.84
6
9
20°C, 60 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0; 25°C, 10 min, stable in this range, rapid loss of activity below pH 6.0 and above pH 9.0
Rhodococcus rhodochrous