Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Li, W.Z.; Zhang, Y.Q.; Yang, H.F.
    Formation and purification of nitrile hydratase from Corynebacterium pseudodiphteritium ZBB-41 (1992), Appl. Biochem. Biotechnol., 36, 171-181.
No PubMed abstract available

Application

EC Number Application Comment Organism
4.2.1.84 synthesis useful for acrylamide production Corynebacterium pseudodiphtheriticum

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.84 Ag+ strong inhibition Corynebacterium pseudodiphtheriticum
4.2.1.84 Cu2+ strong inhibition Corynebacterium pseudodiphtheriticum
4.2.1.84 EDTA strong inhibition Corynebacterium pseudodiphtheriticum
4.2.1.84 Hg2+ strong inhibition Corynebacterium pseudodiphtheriticum
4.2.1.84 iodoacetic acid
-
 Corynebacterium pseudodiphtheriticum
4.2.1.84 NaCN non-competitive inhibition Corynebacterium pseudodiphtheriticum
4.2.1.84 NaN3
-
 Corynebacterium pseudodiphtheriticum
4.2.1.84 phenylmercuric acetate strong inhibition Corynebacterium pseudodiphtheriticum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 0.21
-
 acrylonitrile
-
 Corynebacterium pseudodiphtheriticum
4.2.1.84 210
-
 acrylonitrile pH 7.5, 25°C Corynebacterium pseudodiphtheriticum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.84 25000
-
 x * 25000 + x * 28000, SDS-PAGE Corynebacterium pseudodiphtheriticum
4.2.1.84 28000
-
 x * 25000 + x * 28000, SDS-PAGE Corynebacterium pseudodiphtheriticum
4.2.1.84 80000
-
 gradient gel electrophoresis Corynebacterium pseudodiphtheriticum

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Corynebacterium pseudodiphtheriticum
-
-
-
4.2.1.84 Corynebacterium pseudodiphtheriticum ZBB-41
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 to homogeneity, using several chromatographic steps, including DEAE, hydroxyapatite, and Sephadex G-200 column chromatography Corynebacterium pseudodiphtheriticum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.84 additional information
-
 effect of different organic and inorganic compounds on activity Corynebacterium pseudodiphtheriticum
4.2.1.84 315.2
-
-
 Corynebacterium pseudodiphtheriticum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 acrylonitrile + H2O
-
 Corynebacterium pseudodiphtheriticum 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 acrylonitrile + H2O
-
 Corynebacterium pseudodiphtheriticum ZBB-41 2-propenoic acid amide i.e. acrylic acid amide ?

Subunits

EC Number Subunits Comment Organism
4.2.1.84 ? x * 25000 + x * 28000, SDS-PAGE Corynebacterium pseudodiphtheriticum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.84 25
-
-
 Corynebacterium pseudodiphtheriticum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.84 20
-
 90 min, 60% loss of activity Corynebacterium pseudodiphtheriticum
4.2.1.84 50
-
 90 min, complete loss of activity Corynebacterium pseudodiphtheriticum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7.5
-
-
 Corynebacterium pseudodiphtheriticum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.1.84 6.5 8 25°C, 90 min, stable Corynebacterium pseudodiphtheriticum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.2.1.84 0.13
-
 NaCN pH 7.5, 25°C, non-competitive with acrylonitrile Corynebacterium pseudodiphtheriticum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.84 Corynebacterium pseudodiphtheriticum polyacrylamide isoelectrofocusing
-
 4.2