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Literature summary extracted from

  • Bui, K.; Fradet, H.; Arnaud, A.; Galzy, P.
    A nitrile hydratase with a wide substrate spectrum produces by a Brevibacterium sp. (1984), J. Gen. Microbiol., 130, 89-93.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.84 additional information
-
 additional information values for 12 different substrates, affinity increases as the side chain of the substrate becomes longer Brevibacterium sp.
4.2.1.84 25
-
 acetonitrile pH 7, 30°C Brevibacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.84 Brevibacterium sp.
-
-
-
4.2.1.84 Brevibacterium sp. R312
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.84 cell-free extract preparation Brevibacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.84 2-aminopropionitrile + H2O 90% of the activity with propionitrile Brevibacterium sp. 2-aminopropionic acid amide
-
 ?
4.2.1.84 2-hydroxypropionitrile + H2O i.e. DL-lactonitrile Brevibacterium sp. 2-hydroxypropionic acid amide
-
 ?
4.2.1.84 2-hydroxypropionitrile + H2O 116% of the activity with propionitrile Brevibacterium sp. 2-hydroxypropionic acid amide
-
 ?
4.2.1.84 3-aminopropionitrile + H2O 2.7% of the activity with propionitrile Brevibacterium sp. 3-aminopropionic acid amide
-
 ?
4.2.1.84 3-hydroxypropionitrile + H2O 35% of the activity with propionitrile Brevibacterium sp. 3-hydroxypropanamide
-
 ?
4.2.1.84 acetonitrile + H2O 10% of the activity with propionitrile Brevibacterium sp. acetamide
-
 ?
4.2.1.84 acetonitrile + H2O 10% of the activity with propionitrile Brevibacterium sp. R312 acetamide
-
 ?
4.2.1.84 acrylonitrile + H2O
-
 Brevibacterium sp. 2-propenoic acid amide i.e. acrylic acid amide ?
4.2.1.84 benzonitrile + H2O 1.3% of the activity with propionitrile Brevibacterium sp. benzoic acid amide
-
 ?
4.2.1.84 cyanide + H2O 2% of the activity with propionitrile Brevibacterium sp. formamide
-
 ?
4.2.1.84 isobutyronitrile + H2O 113% of the activity with propionitrile Brevibacterium sp. isobutyric acid amide
-
 ?
4.2.1.84 isobutyronitrile + H2O 113% of the activity with propionitrile Brevibacterium sp. R312 isobutyric acid amide
-
 ?
4.2.1.84 n-butyronitrile + H2O 33% of the activity with propionitrile Brevibacterium sp. n-butyric acid amide
-
 ?
4.2.1.84 n-butyronitrile + H2O 33% of the activity with propionitrile Brevibacterium sp. R312 n-butyric acid amide
-
 ?
4.2.1.84 pivalonitrile + H2O 5.3% of the activity with propionitrile Brevibacterium sp. 2,2-dimethylpropionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Brevibacterium sp. propionic acid amide
-
 ?
4.2.1.84 propionitrile + H2O
-
 Brevibacterium sp. R312 propionic acid amide
-
 ?
4.2.1.84 valeronitrile + H2O 2.7% of the activity with propionitrile Brevibacterium sp. n-pentanoic acid amide
-
 ?
4.2.1.84 valeronitrile + H2O 2.7% of the activity with propionitrile Brevibacterium sp. R312 n-pentanoic acid amide
-
 ?

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.84 30 35 optimum temperature for acetonitrile hydrolysis Brevibacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.84 7
-
-
 Brevibacterium sp.