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Literature summary extracted from

  • Maruyama, K.
    Enzymes responsible for degradation of 4-oxalmesaconic acid in Pseudomonas ochraceae (1983), J. Biochem., 93, 567-574.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.83 HgCl2 partially reversed by DTT Pseudomonas straminea
4.2.1.83 PCMB partially reversed by DTT Pseudomonas straminea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.83 55000
-
 gel filtration Pseudomonas straminea

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.83 Pseudomonas straminea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.83 partial Pseudomonas straminea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.83 culture condition:phthalate-grown cell
-
 Pseudomonas straminea
-

Storage Stability

EC Number Storage Stability Organism
4.2.1.83 -20°C, enzyme gradually inactivated, complete loss of activity after 6 months Pseudomonas straminea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.83 (E)-3-carboxy-5-oxo-2-hex-2-enedioic acid + H2O
-
 Pseudomonas straminea 2-hydroxy-4-oxobutane-1,2,4-tricarboxylate i.e. 4-carboxy-4-hydroxy-2-oxoadipic acid, gamma-oxalcitramalate ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.83 24
-
 assay at Pseudomonas straminea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.83 8.2
-
-
 Pseudomonas straminea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.83 7.4 9.1 50% of activity maximum at pH 7.4 and 9.1 Pseudomonas straminea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.83 Pseudomonas straminea focusing at 6°C
-
 5.1